Therapeutic Effect of Chenodeoxycholic Acid in an Experimental Rabbit Model of Osteoarthritis.
Therapeutic Effect of Chenodeoxycholic Acid in an Experimental Rabbit Model of Osteoarthritis.
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鹅去氧胆酸对实验性兔骨关节炎模型的治疗作用
DOI：
10.1155/2015/780149
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发表时间：
2015
影响因子：
4.6
通讯作者：
He CY
中科院分区：
文献类型：
--
作者：
Yan ZW;Dong J;Qin CH;Zhao CY;Miao LY;He CY
骨关节炎(OA)是一种缓慢进展的关节疾病，常见于中老年人。目前还没有理想的治疗OA的药物。鹅去氧胆酸（CDCA）是动物胆汁中的主要活性成分。然而，CDCA对OA严重程度的治疗作用在很大程度上是未知的。本研究的目的是评价关节内注射CDCA对兔骨性关节炎模型的治疗效果。实验兔采用前交叉韧带横断法（ACLT）诱导骨性关节炎，然后每周1次关节内注射CDCA (10 mg/kg或50 mg/kg)，连续5周。结果显示，CDCA能显著降低股骨髁表面软骨降解，减轻关节软骨和滑膜的病理改变。微ct显示，CDCA还能显著减少骨破坏和关节侵蚀。CDCA能显著降低滑液中基质金属蛋白酶-1 （MMP-1）、基质金属蛋白酶-3 （MMP-3）、白细胞介素-1β (IL-1β)和前列腺素E2 （PGE2）的释放。这些观察结果表明，CDCA可能是OA的潜在治疗剂。
Osteoarthritis (OA) is a slowly progressive joint disease typically seen in middle-age to elderly people. At present, there is no ideal agent to treat OA. Chenodeoxycholic acid (CDCA) was a principal active constituent from animal bile. However, the therapeutic effect of CDCA on OA severity was largely unknown. The purpose of this study was to evaluate the therapeutic effect of intra-articular injection of CDCA in a rabbit OA model. OA was induced in experimental rabbits by anterior cruciate ligament transection (ACLT) and then rabbits were intra-articularly injected with CDCA (10 mg/kg or 50 mg/kg) once per week for 5 weeks. The results showed that CDCA significantly decreased cartilage degradation on the surface of femoral condyles, reducing the pathological changes of articular cartilage and synovial membrane by macroscopic and histological analysis. CDCA also significantly decreased bone destruction and erosion of joint evaluated by micro-CT. Furthermore, CDCA could markedly reduce the release of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-3 (MMP-3), interleukin-1β (IL-1β), and prostaglandin E2 (PGE2) in synovial fluid. These observations highlight CDCA might be a potential therapeutic agent for OA.