Development of sustained antimicrobial-release systems using poly(2-hydroxyethyl methacrylate)/trimethylolpropane trimethacrylate hydrogels
Development of sustained antimicrobial-release systems using poly(2-hydroxyethyl methacrylate)/trimethylolpropane trimethacrylate hydrogels
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DOI：
10.1016/j.actbio.2014.06.016
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发表时间：
2014-10-01
影响因子：
9.7
通讯作者：
Imazato, Satoshi
中科院分区：
文献类型：
--
作者：
Kitagawa, Haruaki;Takeda, Kahoru;Imazato, Satoshi
具有持续抗微生物作用的重建材料可用于在含有固有细菌或真菌的环境（例如口腔）中预防传染病。为了将不可生物降解的水凝胶应用于树脂基材料作为水溶性抗菌剂的储库，制备了由甲基丙烯酸2-羟乙酯（HEMA）和三甲基丙烯酸异丙酯（TMPT）组成的新型水凝胶。将氯化十六烷基吡啶（CPC）负载到包含不同比例的HEMA/TMPT的五种水凝胶中，并在体外检查它们的释放能力以及与CPC再充电的能力。包含50%HEMA/50%TMPT的聚HEMA/TMPT水凝胶可以通过浸入CPC溶液中而有效地负载CPC并再装填CPC，证明CPC的最长释放，高于抑制细菌和真菌所需的浓度。CPC与水凝胶的结合主要是通过疏水相互作用实现的。通过在聚合之前将CPC粉末与HEMA/TMPT单体混合将CPC装载到水凝胶中导致初始CPC释放期的显著延长。通过琼脂扩散试验证实CPC预混合水凝胶具有抗菌活性。通过将CPC预混合加载并再填充到50%HEMA/50%TMPT水凝胶中，可以实现抗菌剂的持续释放系统。(C)2014 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Reconstructive materials with sustained antimicrobial effects could be useful for preventing infectious diseases in an environment containing indigenous bacteria or fungi such as the oral cavity. With the objective of applying a non-biodegradable hydrogel to resin-based materials as a reservoir for water-soluble antimicrobials, novel hydrogels consisting of 2-hydroxyethyl methacrylate (HEMA) and trimethylolpropane trimethacrylate (TMPT) were fabricated. Cetylpyridinium chloride (CPC) was loaded into five hydrogels comprising different ratios of HEMA/TMPT, and their ability to release as well as to be recharged with CPC was examined in vitro. A polyHEMA/TMPT hydrogel comprising 50% HEMA/50% TMPT could be effectively loaded and recharged with CPC by immersion into a CPC solution, demonstrating the longest release of CPC, above the concentration required to inhibit bacteria and fungi. The binding of CPC to the hydrogels was mainly through hydrophobic interaction. Loading of CPC into a hydrogel by mixing CPC powder with the HEMA/TMPT monomer before polymerization resulted in marked extension of the initial CPC-release period. The CPC-pre-mixed hydrogel was confirmed to exhibit antibacterial activity by agar diffusion tests. It is possible to achieve a sustained release system for antimicrobials by pre-mix loading and recharging CPC into a 50% HEMA/50% TMPT hydrogel. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.