Rapid characterization of the chemical constituents of Cortex Fraxini by homogenate extraction followed by UHPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry and GC-MS
Rapid characterization of the chemical constituents of Cortex Fraxini by homogenate extraction followed by UHPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry and GC-MS
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DOI：
10.1002/jssc.201600851
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发表时间：
2016-11-01
影响因子：
3.1
通讯作者：
Zhao, Chunjie
中科院分区：
文献类型：
--
作者：
Wang, Yinan;Han, Fei;Zhao, Chunjie
黄皮是一种重要的中药。本研究采用快速、可靠的匀浆提取方法，并利用响应面法对该方法进行优化。建立了傅里叶变换离子回旋共振质谱联用、气相色谱联用质谱联用的高效液相色谱法分离表征白皮药材成分。液相色谱分离采用C-18色谱柱（150 mm × 2.1 mm, 1.8 μ m），气相色谱分离采用5%苯基-甲基聚硅氧烷固定相（30 m × 0.25 mm × 0.25 mm），注入硅氧烷化样品。结果表明，用液相色谱-质谱法对33个化合物进行了表征，用气相色谱-质谱法对11个化合物进行了表征，其中香豆素是气相色谱-质谱法和液相色谱-质谱法对其进行表征的主要成分。匀浆提取是一种高效、快速的提取方法，香豆素、苯乙醇苷、环烯醚萜苷、苯丙素和木脂素是白蜡皮的主要成分。本研究为进一步研究白蜡皮奠定了基础，也为其他中药成分的快速提取和鉴定提供了依据。
Cortex Fraxini is an important traditional Chinese medicine. In this work, a rapid and reliable homogenate extraction method was applied for the fast extraction for Cortex Fraxini, and the method was optimized by response surface methodology. Ultra high performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry and gas chromatography with mass spectrometry were established for the separation and characterization of the constituents of Cortex Fraxini. Liquid chromatography separation was conducted on a C-18 column (150 mm x 2.1 mm, 1.8 mu m), and gas chromatography separation was performed on a capillary with a 5% phenyl-methylpolysiloxane stationary phase (30 m x 0.25 mm x 0.25 mm) by injection of silylated samples. According to the results, 33 chemical compounds were characterized by liquid chromatography with mass spectrometry, and 11 chemical compounds were characterized by gas chromatography with mass spectrometry, and coumarins were the major components characterized by both gas chromatography with mass spectrometry and liquid chromatography with mass spectrometry. The proposed homogenate extraction was an efficient and rapid method, and coumarins, phenylethanoid glycosides, iridoid glycosides, phenylpropanoids, and lignans were the main constituents of Cortex Fraxini. This work laid the foundation for further study of Cortex Fraxini and will be helpful for the rapid extraction and characterization of ingredients in other traditional Chinese medicines.