HYPOMETHYLATION OF DNA FROM BENIGN AND MALIGNANT HUMAN-COLON NEOPLASMS
HYPOMETHYLATION OF DNA FROM BENIGN AND MALIGNANT HUMAN-COLON NEOPLASMS
复制标题
DOI：
10.1126/science.2579435
复制
发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
FEINBERG, AP
中科院分区：
文献类型：
--
作者：
GOELZ, SE;VOGELSTEIN, B;FEINBERG, AP
用限制性内切酶分析方法测定了显示肿瘤生长的人结肠组织DNA的甲基化状态。与邻近正常组织的DNA相比，良性结肠息肉和恶性癌的DNA都明显低甲基化。用探针检测生长激素，伽马。球蛋白,.alpha。-绒毛膜促性腺激素和。在所有23例肿瘤生长中均检测到甲基化变化。良性息肉的低甲基化程度与恶性组织相似。低甲基化显然是人类结肠肿瘤的一致生化特征，是恶性肿瘤发生前DNA的改变。
The methylation state of DNA from human colon tissue displaying neoplastic growth was determined by means of restriction endonuclease analysis. When compared to DNA from adjacent normal tissue, DNA from both benign colon polyps and malignant carcinomas was substantially hypomethylated. With the use of probes for growth hormone, .gamma.-globin, .alpha.-chorionic gonadotropin and .gamma.-crystallin, methylation changes were detected in all 23 neoplastic growths examined. Benign polyps were hypomethylated to a degree similar to that in malignant tissue. Hypomethylation evidently is a consistent biochemical characteristic of human colonic tumors and is an alteration in the DNA that precedes malignancy.