Role of JAK2 signal transductional pathway in activation and survival of human peripheral eosinophils by interferon‐gamma (IFN‐γ)
Role of JAK2 signal transductional pathway in activation and survival of human peripheral eosinophils by interferon‐gamma (IFN‐γ)
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JAK2信号转导通路在干扰素γ（IFN-γ）激活人外周嗜酸性粒细胞和存活中的作用
DOI：
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发表时间：
1999
影响因子：
4.6
通讯作者：
Tomioka
中科院分区：
文献类型：
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作者：
Ochiai;Tanabe;Ishihara;Kagami;Sugiyama;Sueishi;Koya;Tomioka
本研究的目的是确定JAK通路是否通过人外周嗜酸性粒细胞中的IFN-γ受体信号传导参与嗜酸性粒细胞活化和存活。通过抗CD 16磁珠阴性选择从6名特应性疾病患者的血液中纯化嗜酸性粒细胞。IFN-γ显著上调24-48 h培养的嗜酸性粒细胞的存活率和CD 69表达。 此外，IFN-γ诱导嗜酸性粒细胞中JAK 2的酪氨酸磷酸化，如蛋白质印迹分析所示。最后，特异性JAK 2抑制剂AG-490抑制JAK 2的酪氨酸磷酸化、IFN-γ诱导的嗜酸性粒细胞存活和CD 69表达。总之，这些结果表明IFN-γ通过激活外周嗜酸性粒细胞中的JAK 2诱导嗜酸性粒细胞存活和CD 69表达，表明JAK 2可能通过IFN-γ-IFN-γR相互作用在嗜酸性粒细胞调节中发挥重要作用。
The purpose of this study was to determine whether the JAK pathway is involved in eosinophil activation and survival through IFN‐γ receptor signalling in human peripheral eosinophils. Eosinophils were purified from the blood of six atopic disease patients by anti‐CD16 magnetic bead‐negative selection. IFN‐γ significantly up‐regulated survival and CD69 expression in 24–48 h cultured eosinophils. Further, IFN‐γ induced tyrosine phosphorylation of JAK2 in eosinophils, as indicated by Western blot analysis. Finally, the specific JAK2 inhibitor AG‐490 inhibited the tyrosine phosphorylation of JAK2, IFN‐γ‐induced survival and CD69 expression in eosinophils. In conclusion, these results indicate that IFN‐γ induces eosinophil survival and CD69 expression through the activation of JAK2 in peripheral eosinophils, suggesting that JAK2 may play a significant role in eosinophil regulation by IFN‐γ–IFN‐γR interaction.
影响因子：
4.4
作者：
Yi-Ju Lee;E. Benveniste
通讯作者：
Yi-Ju Lee;E. Benveniste
嗜酸性粒细胞中 IL-5 激活 Jak-STAT 1 信号通路。
DOI：
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发表时间：
1995
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Pazdrak,K;Stafford,S;Alam,R
通讯作者：
Alam,R