SPINE high-throughput crystallization, crystal imaging and recognition techniques: current state, performance analysis, new technologies and future aspects
SPINE high-throughput crystallization, crystal imaging and recognition techniques: current state, performance analysis, new technologies and future aspects
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DOI：
10.1107/s090744490602943x
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发表时间：
2006-10-01
影响因子：
2.2
通讯作者：
Messerschmidt, Albrecht
中科院分区：
文献类型：
--
作者：
Berry, Ian M.;Dym, O.;Messerschmidt, Albrecht
本文回顾了自2002年10月欧洲结构蛋白质组学(SPINE)项目工作包4的范围内高通量和纳升尺度的蛋白质结晶学技术的发展。通过调查欧洲各地脊柱合作伙伴对新技术的吸收、使用和经验，可以看到一幅非常成功地采用了革新结构生物学领域的新工作方法的图景。最后，对结晶方法学的未来发展进行了展望。
This paper reviews the developments in high- throughput and nanolitre- scale protein crystallography technologies within the remit of workpackage 4 of the Structural Proteomics In Europe ( SPINE) project since the project's inception in October 2002. By surveying the uptake, use and experience of new technologies by SPINE partners across Europe, a picture emerges of highly successful adoption of novel working methods revolutionizing this area of structural biology. Finally, a forward view is taken of how crystallization methodologies may develop in the future.