Identification of tyrosine hydroxylase gene expression in rat spleen.
Identification of tyrosine hydroxylase gene expression in rat spleen.
复制标题
大鼠脾脏中酪氨酸羟化酶基因表达的鉴定。
DOI：
10.1016/s0304-3940(01)02126-7
复制
发表时间：
2001
影响因子：
2.5
通讯作者：
Kvetnanský,R
中科院分区：
文献类型：
--
作者：
Kubovcáková,L;Micutková,L;Sabban,EL;Krizanová,O;Kvetnanský,R
本研究旨在鉴定基础和应激条件下大鼠脾脏中酪氨酸羟化酶（TH）基因的表达。利用逆转录聚合酶链反应，我们在对照组和固定动物的大鼠脾脏中均未检测到TH mRNA。半巢式PCR显示了一个清晰的信号，表明TH mRNA在脾脏中形成，尽管丰度较低。我们还检测到大鼠脾脏中TH免疫反应蛋白和TH活性的丰度高于mRNA水平的预期。本研究首次确定了TH基因在大鼠脾脏中的表达。由于TH蛋白及其活性在脾脏中的丰度远高于相应的mRNA，因此大部分TH蛋白很可能是由脾脏交感神经支配提供的。
This study was aimed to identify tyrosine hydroxylase (TH) gene expression in the rat spleen under basal and stress conditions. Using the reverse transcription polymerase chain reaction we did not detect TH mRNA in rat spleen either in control, or immobilized animals. Semi-nested PCR revealed a clear signal, demonstrating that TH mRNA is formed in the spleen, although in low abundance. We also detected both, TH immunoreactive protein and TH activity in the rat spleen that were in higher abundance than expected from the mRNA levels. This study identifies, for the first time, TH gene expression in rat spleen. Since TH protein and activity are present in the spleen in much higher abundance compared to corresponding mRNA, the majority of TH protein is most probably supplied by the sympathetic innervation of spleen.