Apoptotic effect of red wine polyphenols on human colon cancer SNU-C4 cells
Apoptotic effect of red wine polyphenols on human colon cancer SNU-C4 cells
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DOI：
10.1016/j.fct.2005.08.031
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发表时间：
2006-06-01
影响因子：
4.3
通讯作者：
Kim, H. -K.
中科院分区：
文献类型：
--
作者：
Kim, M. -J.;Kim, Y. -J.;Kim, H. -K.
水果、大豆、蔬菜、草药、根和叶中的多酚是与预防癌症、心脏病和糖尿病有关的生物活性成分。采用四甲基偶氮唑盐(3-(4，5-dimethylthiazol-2-yl)-2，5-diphenyltetrazolium)比色法、4，6-二氨基-2-苯基吲哚(DAPI)染色、末端脱氧核苷酸转移酶(TdT)介导的dUTP缺口末端标记法(TUNEL)、逆转录聚合酶链式反应(RT-PCR)检测Bcl2、Bax和Caspase-3基因的表达及Caspase-3酶活性，研究红酒多酚对人结肠癌细胞株SNU-C4的诱导凋亡作用。多酚(100 mU g/ml)可促进SNU-C4细胞的凋亡，DAPI染色和TUNEL法显示明显的细胞凋亡特征，包括染色质凝集和凋亡小体形成的形态改变。与未经处理的对照组相比，多酚(100 mU g/ml)可降低Bcl2的表达，增加Bax和Caspase 3的表达。多酚处理组Caspase-3活性较对照组显著升高(P<；0.05)。这些结果表明，多酚作为一种抗结肠癌药物具有很强的开发潜力。(C)2005爱思唯尔有限公司。保留所有权利。
Polyphenols in fruits, soybean, vegetables, herbs, roots and leaves act as bioactive components related with prevention of cancer, heart diseases and diabetes. We investigated the apoptotic effects of polyphenols from red wine on human colon cancer cells SNU-C4 using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR) expressions of Bcl-2, Bax and Caspase-3 genes, and Caspase-3 enzyme activity. Polyphenols (100 mu g/ml) increased the apoptosis of SNU-C4 cells with apparent apoptotic characteristics including morphological changes of chromatin condensation and apoptotic body formation from DAPI staining and TUNEL assay. Compared with untreated control group, polyphenols (100 mu g/ml) reduced the expression of Bcl-2 whereas those of Bax and Caspase-3 were increased. The Caspase-3 activity in the polyphenols treated group was significantly increased compared to those in control group (P < 0.05). These results suggest that polyphenols have a strong potential for development as an anti-colon cancer agent. (c) 2005 Elsevier Ltd. All rights reserved.