The beta-oxidation pathway is downregulated during diapause termination in Calanus copepods
The beta-oxidation pathway is downregulated during diapause termination in Calanus copepods
复制标题
桡足类滞育终止期间β-氧化途径下调
DOI：
10.1038/s41598-019-53032-5
复制
发表时间：
2019
影响因子：
4.6
通讯作者：
Olsen, RE
中科院分区：
文献类型：
--
作者：
Skottene, E;Tarrant, AM;Olsen, AJ;Altin, D;Østensen, M-A;Hansen, BH;Choquet, M;Jenssen, BM;Olsen, RE
哲水蚤是海洋生态系统中的关键物种，主要是由于它们的高脂质含量，这是幼鱼等的营养食物来源。积累的脂质被分解代谢以满足休眠（滞育）期间的能量需求，这发生在最后的桡足虫阶段（C5）。目前对滞育终止过程中脂类降解途径的认识还很有限。我们的特点是脂质丰满度的变化，并在C5的滞育终止和向成年的进展过程中产生的转录谱。C5的脂质充满度在发育过程中线性下降，但与晚期C5和成人相比，早期C5中更多的β-氧化基因上调。我们确定了四种可能的能量代谢主调节因子，与晚期C5和成人相比，它们在早期C5中普遍上调。我们发现，肉毒碱穿梭中的两种酶之一是不存在的哲水蚤桡足类谱系。根据采样点的地理位置，初步推测野外样品由C. finmarchicus。然而，C.一些样本中的冰川作用强调了进行分子分析以可靠地鉴定哲水蚤物种的必要性。我们的研究结果有助于更好地了解哲水蚤桡足类滞育和滞育终止过程中发生的分子事件。
Calanuscopepods are keystone species in marine ecosystems, mainly due to their high lipid content, which is a nutritious food source for e.g. juvenile fish. Accumulated lipids are catabolized to meet energy requirements during dormancy (diapause), which occurs during the last copepodite stage (C5). The current knowledge of lipid degradation pathways during diapause termination is limited. We characterized changes in lipid fullness and generated transcriptional profiles in C5s during termination of diapause and progression towards adulthood. Lipid fullness of C5s declined linearly during developmental progression, but more β-oxidation genes were upregulated in early C5s compared to late C5s and adults. We identified four possible master regulators of energy metabolism, which all were generally upregulated in early C5s, compared to late C5s and adults. We discovered that one of two enzymes in the carnitine shuttle is absent from the calanoid copepod lineage. Based on the geographical location of the sampling site, the field-samples were initially presumed to consist ofC. finmarchicus. However, the identification ofC. glacialisin some samples underlines the need for performing molecular analyses to reliably identifyCalanusspecies. Our findings contributes to a better understanding of molecular events occurring during diapause and diapause termination in calanoid copepods.