Exogenous sucrose treatment accelerates postharvest tomato fruit ripening through the influence on its metabolism and enhancing ethylene biosynthesis and signaling
Exogenous sucrose treatment accelerates postharvest tomato fruit ripening through the influence on its metabolism and enhancing ethylene biosynthesis and signaling
复制标题
外源蔗糖处理通过影响代谢和增强乙烯生物合成和信号传导来加速采后番茄果实成熟
DOI：
10.1007/s11738-016-2240-5
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发表时间：
2016-09-01
影响因子：
2.6
通讯作者：
Luo, Zisheng
中科院分区：
文献类型：
--
作者：
Li, Dongdong;Mou, Wangshu;Luo, Zisheng
蔗糖在植物中作为信号分子的作用长期以来一直存在争议，直到最近才逐渐被人们所接受。蔗糖在植物的许多发育过程中发挥着重要作用，但其在果实成熟中的作用还没有得到很好的阐明。在这项研究中，外源蔗糖处理（500 mM）对采后番茄果实成熟的影响进行了研究。结果发现，与甘露醇处理（500 mM，设置为对照）相比，蔗糖加速了成熟过程，具有较高水平的呼吸速率和乙烯产生在存储过程中。蔗糖处理上调了果实生物合成基因的表达，同时刺激了果实降解相关酶基因的表达。然而，较高的蔗糖含量观察到蔗糖处理的水果只有在最初的几天。此外，蔗糖的施用对其降解产物葡萄糖和果糖的含量影响不大。此外，外源蔗糖处理上调了乙烯生物合成相关基因的表达，并以不同方式影响乙烯信号转导途径中的关键基因，从而促进了乙烯信号转导。这些结果表明，蔗糖促进番茄果实成熟可能是通过调节自身的代谢，促进营养物质的流动和代谢信号分子的激活，同时也通过促进乙烯的合成和信号转导。
The role of sucrose as a signal molecule in plants was in debate for a long time, until recently, it gradually becomes more prominently accepted. Sucrose plays roles in a vast array of developmental processes in plants, however, its function in fruit ripening has not been well elucidated. In this study, the influence of exogenous sucrose treatment (500 mM) on postharvest tomato fruit ripening was investigated. It was found that, in comparison with mannitol treatment (500 mM, set as control), sucrose accelerated the ripening process with higher levels of respiration rate and ethylene production during the storage. Sucrose treatment up-regulated its biosynthetic genes, whilst stimulated expressions of genes encoding degradation related enzymes in the fruits. However, higher sucrose content was observed in sucrose-treated fruits only in the first few days. In addition, sucrose application had minor effect on the contents of its degrading products, glucose and fructose. Moreover, exogenous sucrose treatment up-regulated expressions of ethylene biosynthetic genes, and promoted ethylene signal transduction via influencing critical genes of the signaling pathway in different patterns. These results indicate that sucrose stimulates tomato fruit ripening may through mediating its own metabolism, which facilitates nutrients fluxes and metabolic signaling molecules activation, and also by enhancing ethylene biosynthesis and signal transduction.