The Effect of Layer-by-Layer Assembly Coating on the Proliferation and Differentiation of Neural Stem Cells
The Effect of Layer-by-Layer Assembly Coating on the Proliferation and Differentiation of Neural Stem Cells
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DOI：
10.1021/am504456t
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发表时间：
2015-02-11
影响因子：
9.5
通讯作者：
Kong, Jiming
中科院分区：
文献类型：
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作者：
Li, Wenyan;Guan, Teng;Kong, Jiming
用生物相容性材料对单细胞进行纳米包覆，为细胞分化和增殖创造了一个确定的微环境，也为细胞生物学研究提供了一个模型。此外，中风患者组织中的酸性环境需要在pH刺激下释放药物。在这里，我们报告的封装单个神经干细胞（NSCs）使用一层一层（LBL）自组装技术与聚电解质明胶和海藻酸盐。对NSC的分析表明，LbL包封不会影响细胞的活力、增殖或分化。将胰岛素样生长因子-1（IGF-1）负载于海藻酸钠涂层材料上，其从海藻酸钠释放到培养基中呈现时间依赖性和pH依赖性。IGF-1显著增强了包封的NSC的增殖，证明了LbL单细胞纳米涂层的药物载体功能。它提供了一个潜在的治疗策略，神经系统疾病，如中风。
Nanocoating of a single-cell with biocompatible materials creates a defined microenvironment for cell differentiation and proliferation, as well as a model for studies in cell biology. In addition, the acidic environment in the tissue of stroke victims necessitates drug release upon pH stimuli. Here, we report the encapsulation of single neural stem cells (NSCs) using a layer-by-layer (LbL) self-assembly technique with polyelectrolytes gelatin and alginate. Analysis of the NSCs showed that the LbL encapsulation would not affect the viability, proliferation, or differentiation of the cells. When insulin-like growth factor-1 (IGF-1) was loaded on the coating material alginate, its release from alginate into the medium presented in a time-dependent and pH-dependent way. IGF-1 significantly enhanced the proliferation of the encapsulated NSCs, demonstrating a drug-carrier function of the LbL single-cell nanocoating. It provided a potential treatment strategy for nervous system disorders such as stroke.