A NEW BACTERIAL CHLOROPHYLL
A NEW BACTERIAL CHLOROPHYLL
复制标题
DOI：
10.1016/0006-291x(63)90422-4
复制
发表时间：
1963-01-01
影响因子：
3.1
通讯作者：
AASMUNDRUD, O
中科院分区：
文献类型：
--
作者：
EIMHJELLEN, KE;JENSEN, A;AASMUNDRUD, O
迄今为止，已经报道了三种叶绿素实体存在于光合细菌中，即紫色细菌中的细菌叶绿素（货车Niel，1944）和绿色细菌中的叶绿素-660（Larsen，1953）和叶绿素-650（Starrier & Smith，1960）。我们希望报告一个新的叶绿素从一个新分离的光合细菌，这是暂时确定为一个红球藻。（Aasmundrud & Eimhouseen）.新的叶绿素的性质进行了比较，从Rhps获得的细菌叶绿素。球状体。我们建议将已经确定的紫色细菌的叶绿素组分命名为细菌叶绿素2，正如Fischer和Hasenkamp（1935）（Seybold和Hirch，1954）所建议的那样，在本通讯中发现的新叶绿素命名为细菌叶绿素h。Rhps。spheroides（菌株2. 4. 9 c从R博士获得。斯塔尼耶省细菌，加州大学伯克利分校）和新分离的光合细菌在完全填充有由0.5%酵母提取物（D&o）、0.05%MgS 〇 4组成的培养基的玻璃塞瓶中生长。7 H20，M/100-磷酸盐缓冲液（K2 HP 〇 4-NaH 2 P 〇 4）和M/100（NH 4）2-琥珀酸盐的自来水溶液，pH调节至6。S-7.2将培养物在25-28 ℃的光柜中孵育，在最大生长后不久，通过离心收集细胞。在0 - 5 ℃下用甲醇提取浓缩的细胞两次.将一份合并的叶绿素提取物用于制备脱镁叶绿素（货车Niel和Arnold，1938），用乙醚代替四氯化碳作为反应产物的最终溶剂。其余的
Three chlorophyll entities have so far been reported to occur in photosynthetic bacteria, namely bacteriochlorophyll in purple bacteria (van Niel, 1944) and chlorobium chlorophyll-660 (Larsen, 1953) and chlorobium chlorophyll-650 (Starrier & Smith, 1960) in green bacteria. We wish to report on a new chlorophyll from a newly isolated photosynthetic bacterium which is tentatively identified as a Rhodopseudomonas sp.(Aasmundrud & Eimhjellen). The properties of the new chlorophyll have been compared to those of bacteriochlorophyll obtained from Rhps. spheroides. We propose that the already well established chlorophyll component of the purple bacteria be named bacteriochlorophyll 2 as suggested earlier by Fischer and Hasenkamp (1935)(Seybold & Hirch, 1954) and, that the new chlorophyll reportedin the present communication be named bacteriochlorophyll h.Experimental. Rhps. spheroides (strain 2. 4. 9 c obtained from Dr. R. Stanier, Dept. Bact., Univ. California, Berkeley) and the newly isolated photosynthetic bacterium were grown in glass-stoppered bottles completely filled with a medium composed of 0.5% yeast extract (D&o), 0.05% MgS04. 7 H20, M/100-phosphate buffer (K2HP04-NaH2P04) and M/100 (NH4) 2-succmate in tap water, pH adjusted to 6. S-7.2. The cultures were incubated in a light cabinet at 25-28 C, and soon after maximum growth the cells were collected by centrifugation. The packed cells were extracted twice with methanol at 0 to 5 C. An aliquot of the combined chlorophyll extracts was used for preparation of pheophytins (van Niel and Arnold, 1938) substituting diethyl ether for carbon tetrachloride as the final solvent for the reaction products. The rest of the