Sperm quality assessment via separation and sedimentation in a microfluidic device
Sperm quality assessment via separation and sedimentation in a microfluidic device
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DOI：
10.1039/c3an00900a
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发表时间：
2013-01-01
期刊：
影响因子：
4.2
通讯作者：
Wo, Andrew M.
中科院分区：
文献类型：
--
作者：
Chen, Chang-Yu;Chiang, Tsun-Chao;Wo, Andrew M.
不育症的一个主要原因是由于男性因素，包括精子的质量，这是一个主要因素，往往难以评估，特别是总精子浓度及其运动百分比。这项工作提出了一个简单的微流控装置，以评估精子质量，通过量化总精子数和活动精子数。微流控装置的关键设计特征是由渗透相引导结构分开的两个通道，其中一个通道填充有原始精液，另一个填充有纯缓冲液。精液样本在两个腔室中达到平衡，而不活动的精子保留在原始通道中，大约一半的活动精子会游过相位引导屏障进入缓冲通道。离心后，每个通道中的精子聚集成团块，相应的面积代表总精子浓度和活动精子浓度。检测了总精子浓度高达10（8）个精子/ml，活动百分比在10 - 70%范围内，包括世界卫生组织标准规定的40%临界值。离心后，患者样本的结果显示出致密且坚固的颗粒。微流控装置和Makler室之间的总精子浓度的比较显示，它们在5%内一致，并且显示出强相关性，决定系数为R-2 = 0.97。微流体装置和Makler室之间的活动精子计数在5%内一致，决定系数R-2 = 0.84。比较Makler小室、精子质量分析仪和微流控装置的结果表明，微流控装置的结果与Makler小室一致。精子微流控芯片在一次旋转中分析总精子浓度和活动精子浓度，准确且易于使用，并且应该能够轻松进行精子质量分析。
A major reason for infertility is due to male factors, including the quality of spermatozoa, which is a primary factor and often difficult to assess, particularly the total sperm concentration and its motile percentage. This work presents a simple microfluidic device to assess sperm quality by quantifying both total and motile sperm counts. The key design feature of the microfluidic device is two channels separated by a permeative phase-guide structure, where one channel is filled with raw semen and the other with pure buffer. The semen sample was allowed to reach equilibrium in both chambers, whereas non-motile sperms remained in the original channel, and roughly half of the motile sperms would swim across the phase-guide barrier into the buffer channel. Sperms in each channel agglomerated into pellets after centrifugation, with the corresponding area representing total and motile sperm concentrations. Total sperm concentration up to 10(8) sperms per ml and motile percentage in the range of 10-70% were tested, encompassing the cutoff value of 40% stated by World Health Organization standards. Results from patient samples show compact and robust pellets after centrifugation. Comparison of total sperm concentration between the microfluidic device and the Makler chamber reveal they agree within 5% and show strong correlation, with a coefficient of determination of R-2 = 0.97. Motile sperm count between the microfluidic device and the Makler chamber agrees within 5%, with a coefficient of determination of R-2 = 0.84. Comparison of results from the Makler Chamber, sperm quality analyzer, and the microfluidic device revealed that results from the microfluidic device agree well with the Makler chamber. The sperm microfluidic chip analyzes both total and motile sperm concentrations in one spin, is accurate and easy to use, and should enable sperm quality analysis with ease.