Hybridization affects the structure and function of root microbiome by altering gene expression in roots of wheat introgression line under saline-alkali stress
Hybridization affects the structure and function of root microbiome by altering gene expression in roots of wheat introgression line under saline-alkali stress
复制标题
杂交通过改变盐碱胁迫下小麦渗入系根部的基因表达来影响根部微生物组的结构和功能
DOI：
10.1016/j.scitotenv.2022.155467
复制
发表时间：
2022-05-02
影响因子：
9.8
通讯作者：
Li, Fei
中科院分区：
文献类型：
--
作者：
Cui, Ming-Han;Chen, Xiang-Yu;Li, Fei
植物与其根部微生物群之间的互利关系对于植物适应不利环境至关重要。然而，小麦调控根微生物组结构的分子机制以及远缘杂交对这一过程的影响还知之甚少。本研究系统比较了耐盐碱小麦渐渗系SR4（小麦与高冰草体细胞杂交获得）及其亲本小麦品种JN177的根转录组和微生物组。结果表明，根际微生物是维持盐碱胁迫下SR4比JN177更好的钠、钾离子含量动态平衡的关键因素。通过系统比较，我们确定了SR4特定的根细菌和真菌类群在盐碱胁迫下。通过结合微生物组和转录组数据的加权基因相关网络分析（WGCNA），确定了与JN177和SR4之间具有差异丰度的根细菌和真菌密切相关的关键功能基因和途径。这些结果表明，体细胞杂交改变了调控小麦根系微生物组的关键基因，进一步提高了小麦渐渗系的耐盐碱性。这些发现为盐碱胁迫下小麦根系微生物组工程提供了关键的细菌和真菌类群和功能靶基因。
The mutually beneficial relationship between plants and their root microbiota is essential for plants to adapt to unfavorable environments. However, the molecular mechanism of wheat regulating the structure of root microbiome and the influence of distant hybridization on this process are poorly understood. In this study, we systematically compared the root transcriptome and microbiome between a saline-alkali tolerant wheat introgression line SR4 (derived from somatic hybridization between wheat and tall wheatgrass) and its parent wheat variety JN177. The results indicated that root microorganisms were key factor maintaining better homeostasis of the sodium and potassium ion contents in SR4 than in JN177 under saline-alkali stress. Through systematic comparisons, we identified SR4-specific root bacterial and fungal taxa under saline-alkali stress. Through a weighted gene correlation network analysis (WGCNA) combining microbiome and transcriptome data, key functional genes and pathways, which were strongly related to root bacteria and fungi with differential abundance between JN177 and SR4, were identified. These results suggest that somatic hybridization has altered the key genes regulating root microbiome in wheat, further improving the saline-alkali tolerance of wheat introgression line. These findings provide the key bacterial and fungal taxa and functional target genes for wheat root microbiome engineering under saline-alkali stress.