The complete cDNA sequence of a type II Trichomonas vaginalis virus
The complete cDNA sequence of a type II Trichomonas vaginalis virus
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DOI：
10.1006/viro.1999.0129
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发表时间：
2000-02-15
期刊：
影响因子：
3.7
通讯作者：
Tai, JH
中科院分区：
文献类型：
--
作者：
Bessarab, IN;Liu, HW;Tai, JH
阴道毛滴虫病毒（Trichomonasvagulovirus，TVV）是一类具有不同结构的双链RNA病毒，可调控病原体阴道毛滴虫（Trichomonasvagulovirus，TVV）中P270基因的表达。在本研究中，完整的4674 bp的cDNA序列的4.6 kb的双链RNA从一个新确定的TVV 2 -1分离物进行了测定。正链mRNA序列包含4个开放的阅读框，分别在阅读框2和阅读框1编码重叠的cap和pol基因，在第3个阅读框编码两个推定的富含丝氨酸-苏氨酸的碱性蛋白VP 3和VP 4。一个85-kDa的衣壳蛋白和180-kDa的CAP-POL融合蛋白，确定了在粗病毒的蛋白质印迹实验，使用抗血清对基因特异性寡肽。结合cap和pol基因重叠内潜在的核糖体滑七核苷酸G GGC CCC的存在，这些观察结果表明TVV 2 -1的pol基因在cap基因翻译期间通过-1核糖体移码事件进行翻译。我们的研究结果还提供了洞察不同的dsRNA物种之间的保守性从TW和建议TVV 2 -1的基因组可能编码两个额外的基因，除了帽和pol基因。(C)北京大学出版社.
Trichomonas vaginalis viruses (TVV), which may regulate P270 gene expression in the protozoan pathogen T: vaginalis, are a group of divergent double-stranded (ds) RNA viruses. In the present study, the complete 4674-bp cDNA sequence of a 4.6-kb ds RNA from a newly identified TVV2-1 isolate was determined. The sequence of the plus-strand mRNA contains four open reading frames, which encode overlapping cap and pol genes in the reading frame 2 and reading frame 1, respectively, and two putative serine-threonine-rich basic proteins VP3 and VP4 in the third reading frame. An 85-kDa capsid protein and a 180-kDa CAP-POL fusion protein were identified in crude viruses by Western blotting experiments using antisera raised against gene-specific oligopeptides. In conjunction with the presence of a potential ribosomal slippery heptanucleotide G GGC CCC within the overlap of the cap and pol genes, these observations suggest that the pol gene of TVV2-1 is translated via a -1 ribosomal frameshifting event during translation of the cap gene. Our results also provide insight into the conservation among divergent dsRNA species from TW and suggest that the genome of TVV2-1 may encode two extra genes in addition to the cap and pol genes. (C) 2000 Academic Press.