Immunohistochemical Analysis of Cell Cycle Regulatory Gene Products in Normal Trophoblast and Placental Site Trophoblastic Tumor
Immunohistochemical Analysis of Cell Cycle Regulatory Gene Products in Normal Trophoblast and Placental Site Trophoblastic Tumor
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正常滋养细胞和胎盘部位滋养细胞肿瘤细胞周期调节基因产物的免疫组织化学分析
DOI：
10.1097/00004347-199807000-00007
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发表时间：
1998
影响因子：
2.4
通讯作者：
S. Fujii
中科院分区：
文献类型：
--
作者：
N. Ichikawa;Y. Zhai;T. Shiozawa;T. Toki;H. Noguchi;T. Nikaido;S. Fujii
摘要中间滋养细胞很少引起胎盘部位滋养细胞肿瘤。为了研究正常和肿瘤性IT中存在的不同生长机制，比较了正常植入部位和PSTT中细胞周期调节分子的表达。用抗细胞角蛋白、人绒毛膜促性腺激素和人胎盘催乳素的抗体对19例妊娠早期正常着床部位和6例PSTT进行免疫化学研究，以确定IT，并用抗Ki-67、细胞周期蛋白（A、B、D1和E）、细胞周期蛋白依赖性激酶（cdks）和p53的抗体研究滋养层细胞的增殖活性。在细胞柱的滋养层中观察到显著的增殖活性。正常IT显示Ki-67标记指数很低，除cyclins B和E外，cdks和cyclins均阴性表达。PSTT肿瘤细胞Ki-67标记指数高，cyclins和cdks均阳性表达。P53阳性细胞的分布与cyclin A阳性细胞的分布有一定的相关性。PSTT的转化IT具有高增殖活性，细胞周期调节分子的异常表达，这在正常IT中没有观察到。
SummaryIntermediate trophoblast (IT) rarely gives rise to a placental site trophoblastic tumor (PSTT). To examine the different growth mechanisms present in normal and neoplastic IT, the expression of cell cycle regulatory molecules was compared at normal implantation sites and in PSTTs. Normal implantation sites in early gestation (19 patients) and PSTTs (6 patients) were immunohistochemically studied using antibodies against cytokeratin, human chorionic gonadotropin, and human placental lac-togen to identify IT, and antibodies against Ki-67, cyclins (A, B, D1, and E), cyclin-dependent kinases (cdks), and p53 to investigate the proliferative activity of the trophoblast. Marked proliferative activity was observed in the trophoblast of the cell columns. Normal IT exhibited a very low labeling index for Ki-67, with negative expression for cdks and cyclins, except for cyclins B and E. The tumor cells of PSTT exhibited a high labeling index for Ki-67 with positive expression for all the cyclins and cdks examined. Expression of p53 was identified in tumor cells of PSTTs and the distribution of p53-positive cells correlated topographically with that of the cyclin A-positive cells. The transformed IT of PSTT has high proliferative activity with an abnormal expression of cell cycle regulatory molecules, which is not observed in normal IT.