Polymerase chain reaction diagnosis of leishmaniasis: A species-specific approach.
Polymerase chain reaction diagnosis of leishmaniasis: A species-specific approach.
复制标题
利什曼病的聚合酶链反应诊断：一种物种特异性方法。
DOI：
10.1007/978-1-4939-3360-0_11
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Mondolfi AP.
中科院分区：
文献类型：
--
作者：
Gonzalez-Marcano E;Kato H;Concepcion JL;Marquez ME;Mondolfi AP.
利什曼病是一种由利什曼属原生动物寄生虫引起的传染病，通过受感染的沙蝇叮咬传播给人类。不同的临床表现和疾病的演变是由感染物种决定的。在物种水平上的识别是至关重要的，因为这极大地影响了治疗决策以及预测疾病的结果。本章描述了聚合酶链反应（PCR）在整个疾病谱系中检测利什曼原虫的应用，包括样品收集和运输，基因组物质提取和靶扩增方法，特别强调了利什曼原虫物种鉴定的细胞显色基因的PCR扩增。
Leishmaniasis is an infectious disease caused by protozoan parasites of the genusLeishmaniawhich are transmitted to humans through bites of infected sand flies. The variable clinical manifestations and the evolution of the disease are determined by the infecting species. Recognition at a species level is of utmost importance since this greatly impacts therapy decision making as well as predicts outcome for the disease. This chapter describes the application of polymerase chain reaction (PCR) in the detection ofLeishmaniaparasites across the disease spectrum, including protocols for sample collection and transportation, genomic material extraction, and target amplification methods with special emphasis on PCR amplification of the cytochromebgene forLeishmania spp. species identification.