Afterglow Solid-State NMR Spectroscopy.
Afterglow Solid-State NMR Spectroscopy.
复制标题
余辉固体核磁共振波谱。
DOI：
10.1007/978-1-4939-7386-6_3
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Traaseth,NathanielJ
中科院分区：
文献类型：
--
作者：
Abramov,Gili;Traaseth,NathanielJ
生物分子固态NMR实验传统上是通过检测13 C或15 N核来收集的。由于这些核具有相对较低的灵敏度，这是由于它们相对于1H的旋磁比较小，因此收集多维数据集所需的时间限制了共振分配和结构确定。该领域的一个改进是采用同时或并行采集技术，目的是一次采集多个数据集，从而加快整个数据收集过程。这些实验的核心是交叉极化（CP）元件，它是通过磁偶极耦合在原子核之间转移磁化的一种方式。在本章中，我们将展示CP后剩余的残留信号如何成为可用于获取其他数据集的极化源。这类实验的设置，称为afterglowspectroscopy，描述和证明使用膜蛋白转运蛋白参与多药耐药性。
Biomolecular solid-state NMR experiments have traditionally been collected through detection of13C or15N nuclei. Since these nuclei have relatively low sensitivity stemming from their smaller gyromagnetic ratios relative to1H, the time required to collect multi-dimensional datasets serves as a limitation to resonance assignment and structure determination. One improvement in the field has been to employ simultaneous or parallel acquisition techniques with the goal of acquiring more than one dataset at a time and therefore speeding up the overall data collection process. Central to these experiments is the cross-polarization (CP) element, which serves as a way to transfer magnetization between nuclei via magnetic dipolar couplings. In this chapter, we show how residual signal remaining after CP is a polarization source that can be used to acquire additional datasets. The setup of this class of experiments, referred to asAfterglowspectroscopy, is described and demonstrated using a membrane protein transporter involved in multidrug resistance.