A novel bFGF antagonist peptide inhibits breast cancer cell growth
A novel bFGF antagonist peptide inhibits breast cancer cell growth
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新型 bFGF 拮抗肽抑制乳腺癌细胞生长
DOI：
10.3892/mmr.2012.882
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发表时间：
2012-07-01
影响因子：
3.4
通讯作者：
Wu, Xiaoping
中科院分区：
文献类型：
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作者：
Li, Quchou;Gao, Susu;Wu, Xiaoping
乳腺癌是全世界女性中最常见的癌症类型。在乳腺癌患者中发现碱性成纤维细胞生长因子(BFGF)表达升高。我们先前从噬菌体展示的随机七肽库中获得了一个高亲和力的碱性成纤维细胞生长因子结合肽(命名为P7)。在本研究中，我们发现P7多肽能够显著抑制碱性成纤维细胞生长因子刺激的乳腺癌细胞株MDA-MB-231的增殖。另外，实验还揭示了P7肽抑制bFGF刺激的乳腺癌细胞增殖的机制可能与细胞周期停滞于G(O)/G(1)期、阻断ERK和P38信号通路的激活以及上调生长抑制因子增殖相关蛋白2G4的表达有关。这些结果表明，碱性成纤维细胞生长因子结合肽可能在乳腺癌的治疗中具有潜在的治疗潜力。
Breast cancer is the most common type of cancer in women worldwide. Elevated expression of the basic fibroblast growth factor (bFGF) has been found in patients suffering from breast cancer. We previously obtained a high-affinity bFGF-binding peptide (named P7) from a phage-display random heptapeptide library. In this study, we show that P7 peptides significantly inhibits the proliferation of the bFGF-stimulated MDA-MB-231 breast cancer cell line. Additional experiments revealed that the mechanisms of the P7 peptide inhibition of the cell proliferation of breast cancer cells stimulated with bFGF in vitro involved cell cycle arrest at the G(o)/G(1) phase, blockade of the activation of Erk and P38 cascades and the upregulation of the expression of the growth inhibitor, proliferation-associated protein 2G4. These results suggest that the bFGF-binding peptide may have therapeutic potential in breast cancer therapy.