Live imaging of rat embryos with Doppler swept-source optical coherence tomography
Live imaging of rat embryos with Doppler swept-source optical coherence tomography
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DOI：
10.1117/1.3241044
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发表时间：
2009-09-01
影响因子：
3.5
通讯作者：
Larin, Kirill V.
中科院分区：
文献类型：
--
作者：
Larina, Irina V.;Furushima, Kenryo;Larin, Kirill V.
长期以来，大鼠一直被认为是研究哺乳动物胚胎心血管生理学的优秀系统，但缺乏小鼠中能够产生单基因突变的广泛遗传工具。然而，最近建立的大鼠胚胎干细胞系促进了大鼠胚胎中新模型的产生，将生理变化与基因功能改变联系起来，以确定先天性心血管出生缺陷背后的潜在机制。除了创造新的大鼠基因型的能力之外，还强烈需要以高空间和时间分辨率分析表型的工具。多普勒 OCT 此前已用于其他模型物种的 3D 结构分析和血流成像。我们使用多普勒扫频 OCT 对早期植入后大鼠胚胎进行实时成像。结构成像用于胚胎形态的 3D 重建以及跳动心脏和血管的动态成像，而多普勒模式成像用于血流可视化。我们证明，多普勒扫源 OCT 可以提供有关早期大鼠胚胎动力学的重要信息，并作为大鼠胚胎生理学功能评估的广泛研究的基础。 (C) 2009 年光电仪器工程师协会。 [DOI：10.1117/1.3241044]
The rat has long been considered an excellent system to study mammalian embryonic cardiovascular physiology, but has lacked the extensive genetic tools available in the mouse to be able to create single gene mutations. However, the recent establishment of rat embryonic stem cell lines facilitates the generation of new models in the rat embryo to link changes in physiology with altered gene function to define the underlying mechanisms behind congenital cardiovascular birth defects. Along with the ability to create new rat genotypes there is a strong need for tools to analyze phenotypes with high spatial and temporal resolution. Doppler OCT has been previously used for 3-D structural analysis and blood flow imaging in other model species. We use Doppler swept-source OCT for live imaging of early postimplantation rat embryos. Structural imaging is used for 3-D reconstruction of embryo morphology and dynamic imaging of the beating heart and vessels, while Doppler-mode imaging is used to visualize blood flow. We demonstrate that Doppler swept-source OCT can provide essential information about the dynamics of early rat embryos and serve as a basis for a wide range of studies on functional evaluation of rat embryo physiology. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3241044]