Acidiplasma aeolicum gen. nov., sp nov., a euryarchaeon of the family Ferroplasmaceae isolated from a hydrothermal pool, and transfer of Ferroplasma cupricumulans to Acidiplasma cupricumulans comb. nov.
Acidiplasma aeolicum gen. nov., sp nov., a euryarchaeon of the family Ferroplasmaceae isolated from a hydrothermal pool, and transfer of Ferroplasma cupricumulans to Acidiplasma cupricumulans comb. nov.
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DOI：
10.1099/ijs.0.009639-0
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发表时间：
2009-11-01
影响因子：
2.8
通讯作者：
Golyshin, Peter N.
中科院分区：
文献类型：
--
作者：
Golyshina, Olga V.;Yakimov, Michail M.;Golyshin, Peter N.
从意大利武尔卡诺岛的一个热液池中分离到一种新的嗜酸性、无细胞壁的古菌菌株V-T。观察到细胞形态从多形性到球形不等。菌株V-T的生长温度范围为15-65 ℃，最适温度为45 ℃。生长的pH范围为pH 0 - 4，最适pH为1.4-1.6。菌株V-T能够在需氧和厌氧条件下生长，分别氧化亚铁和还原三价铁。该分离物以酵母提取物和以葡萄糖作为能量和碳源的酵母提取物进行化学-有机营养生长。DNA中的G + C摩尔含量为36mol%。16 S rRNA基因序列分析表明，V-T菌株属于广古菌门热原体目铁原体科（Ferroplasmaceae），与铜铁原体BH 2（T）的同源性为100%，与嗜酸铁原体Y-T的同源性为95.4%，与嗜酸菌（Picrophilus torridus DSM 9790（T）的同源性为94%，与大岛嗜酸菌（Picrophilus oshimae DSM 9789（T）的同源性为92%。基于16 S rRNA基因序列的系统发育分析表明，V-T菌株与F. cupricumulans BH 2（T）和所有其它具有可用16 S rRNA基因序列的嗜热分离物，而F acidiphilum Y-T与铁浆菌科内的嗜温分离物形成另一个簇。菌株V-T与F cupricumulans BH 2（T）的DNA-DNA杂交值远低于70%，表明两个菌株属于不同的种。极性脂质主要由基于半乳糖基二联植烷基磷酸甘油四醚的单一磷酸糖脂衍生物，以及少量的单糖基和二糖基二联植烷基醚脂质和相应的磷酸甘油衍生物。主要呼吸醌是萘醌衍生物。考虑到新分离株与铁浆菌属模式种之间显著的生理和化学差异以及不同的系统发育位置，我们建议菌株V-T为一个新属和新种的成员，风酸浆菌新属，模式菌株为V-T（=DSM 18409（T）=JCM 14615（T））。此外，我们建议将铁胞浆菌属的Cupricumulans Hawkes等2008转移到酸胞浆菌属，作为酸胞浆菌属的Cupricumulans comb. nov.（模式菌株BH 2（T）=DSM 16551（T）=JCM 13668（T））。
A novel acidophilic, cell-wall-less archaeon, strain V-T, was isolated from a hydrothermal pool on Vulcano Island, Italy. The morphology of cells was observed to vary from pleomorphic to coccoid. The temperature range for growth of strain V-T was 15-65 degrees C with an optimum at 45 degrees C. The pH for growth ranged from pH 0 to 4 with an optimal at pH 1.4-1.6. Strain V-T was able to grow aerobically and anaerobically, oxidizing ferrous iron and reducing ferric iron, respectively. The isolate grew chemo-organotrophically with yeast extract and yeast extract with glucose as the sources of energy and carbon. The molar G + C content in the DNA was 36 mol%. 16S rRNA gene sequence analysis demonstrated that strain V-T was a member of the family Ferroplasmaceae, order Thermoplasmatales, phylum Euryarchaeota, showing sequence identities of 100 % with Ferroplasma cupricumulans BH2(T), 95.4 % with Ferroplasma acidiphilum Y-T 94% with Picrophilus torridus DSM 9790(T) and 92 % with Picrophilus oshimae DSM 9789(T). 16S rRNA gene sequence-based phylogenetic analysis showed that strain V-T formed a monophyletic cluster together with F. cupricumulans BH2(T) and all other thermophilic isolates with available 16S rRNA gene sequences, whereas F acidiphilum Y-T formed another cluster with mesophilic isolates within the family Ferroplasmaceae. DNA-DNA hybridization values between strain V-T and F cupricumulans BH2(T) were well below 70%, indicating that the two strains belong to separate species, Principal membrane lipids of strain V-T were dibiphytanyl-based tetraether lipids containing pentacyclic rings. The polar lipids were dominated by a single phosphoglycolipid derivative based on a galactosyl dibiphytanyl phosphoglycerol tetraether, together with smaller amounts of monoglycosyl and diglycosyl dibiphytanyl ether lipids and the corresponding phosphoglycerol derivatives. The major respiratory quinones present were naphthoquinone derivatives. Given the notable physiological and chemical differences as well as the distinct phylogenetic placement of the new isolate relative to the type species of the genus Ferroplasma, we propose strain V-T as a member of a new genus and species, Acidiplasma aeolicurn gen. nov., sp. nov. The type strain of Acidiplasma aeolicum is strain V-T (=DSM 18409(T) =JCM 14615(T)). in addition, we propose to transfer Ferroplasma cupricumulans Hawkes et al. 2008 to the genus Acidiplasma as Acidiplasma cupricumulans comb. nov. (type strain BH2(T) =DSM 16551(T) =JCM 13668(T)).