Molecular and genetic characterization ofSLC1, a putativeSaccharomyces cerevisiae homolog of the metazoan cytoplasmic dynein light chain 1
Molecular and genetic characterization ofSLC1, a putativeSaccharomyces cerevisiae homolog of the metazoan cytoplasmic dynein light chain 1
复制标题
DOI：
10.1007/bf02174342
复制
发表时间：
1996-04
期刊：
影响因子：
--
通讯作者：
Thomas Dick;William Chia;Uttam Surana
中科院分区：
文献类型：
--
作者：
Thomas Dick;William Chia;Uttam Surana
细胞质动力蛋白是一种多亚基的微管依赖性运动酶，被认为在细胞内的各种运动中发挥作用。作为努力了解细胞质动力蛋白的进化和生物学作用的一部分，我们已经确定了第一个非后生动物动力蛋白轻链1，SLC 1，在酵母酿酒酵母。SLC1蛋白的氨基酸序列与人、果蝇和Causophila habditiscytoplasmic dynein轻链1的氨基酸序列相似。SLC 1基因位于YAP 2（CAD 1）转录单位的附近。SLC1编码序列被两个内含子分开，其mRNA在整个细胞周期中都可检测到。对SLC 1编码区含有TRP插入的杂合子进行的四分体分析表明，SLC 1的功能对细胞的生存能力不是必需的。此外，我们证明了SLC 1和驱动蛋白相关的CIN 8基因的双突变体是非致死性的。酵母中SLC 1功能的冗余与果蝇中动力蛋白轻链1基因功能缺失突变引起的细胞死亡形成对比。
Cytoplasmic dynein is a multisubunit, microtubule-dependent motor enzyme that has been proposed to function in a variety of intracellular movements. As part of an effort to understand the evolution and the biological roles of cytoplasmic dynein, we have identified the first non-metazoan dynein light chain 1, SLC1, in the yeastSaccharomyces cerevisiae. The amino acid sequence of the SLC1 protein is similar to those of the human,DrosophilaandCaenorhabditiscytoplasmic dynein light chains 1. TheSLC1gene lies adjacent to theYAP2(CAD1) transcription unit. TheSLC1coding sequence is split by two introns and its mRNA is detectable throughout the cell cycle. Tetrad analysis of heterozygotes harboring aTRPinsertion in theSLC1coding region indicate thatSLC1function is not essential for cell viability. Furthermore, we demonstrate that double mutants, defective forSLC1and the kinesin-relatedCIN8genes are non-lethal. The redundancy ofSLC1function in yeast contrasts with the cell death caused by loss-of-function mutations in the dynein light chain 1 gene inDrosophila melanogaster.