Rapid and Directional Improvement of Elite Rice Variety via Combination of Genomics and Multiplex Genome Editing
Rapid and Directional Improvement of Elite Rice Variety via Combination of Genomics and Multiplex Genome Editing
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基因组学与多重基因组编辑相结合快速定向改良优良水稻品种
DOI：
10.1021/acs.jafc.1c08028
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发表时间：
2022
影响因子：
6.1
通讯作者：
Bojun Ma
中科院分区：
文献类型：
--
作者：
Pengcheng Liu;Lumei He;Le Mei;Wenxue Zhai;Xifeng Chen;Bojun Ma
高产、优质是育种工作者追求的主要作物改良目标。然而，这两个性状都是由多个基因控制的复杂农艺性状，因此通过有性重组同时改良这些性状是费时且不受方向控制的。为了解决这一困境，我们引入了基于比较基因组分析的多重基因组编辑系统(CG-MGE)，这是一种快速、定向地改良多个性状的方法。应用该方法对Gn1a、GW2、TGW3和Chalk5进行了表型与表型的关联分析，挖掘出了优良等位基因，并通过多重基因组编辑同时产生了Gn1a、GW2、TGW3和Chalk5等罕见的优良等位基因，成功地改良了一个广为栽培的优良水稻品种的株型结构、产量和品质。总之，本研究为作物的快速定向改良提供了一种方法，CG-MGE的应用将有助于促进合理设计育种。
High yield and superior quality are the main goals pursued by breeders for crop improvement. However, both of them are complex agronomic traits controlled by multiple genes, so the simultaneous improvement of these traits via sexual recombination is time-consuming and direction-uncontrolled. In this study, to solve this dilemma, we introduced the comparative genomic analysis based multiplex genome editing system (CG-MGE), a method for rapid and directional improvement of multiple traits. Application of this method, association analysis between genotypes and phenotypes was carried out to mine excellent alleles; subsequently, the rare excellent alleles of Gn1a, GW2, TGW3, and Chalk5 were simultaneously created by multiplex genome editing and successfully improved the plant architecture, grain yield, and quality of a widely cultivated elite rice variety. Overall, this study provides a method for rapid and directional improvement of crops, and the application of the CG-MGE will be helpful to accelerate rational design breeding.