Aquaporin expression in the human intervertebral disc
Aquaporin expression in the human intervertebral disc
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DOI：
10.1007/s10735-008-9166-1
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发表时间：
2008-06-01
影响因子：
3.2
通讯作者：
Mobasheri, A.
中科院分区：
文献类型：
--
作者：
Richardson, S. M.;Knowles, R.;Mobasheri, A.
髓核（NP）的人椎间盘（IVD）是一个高渗组织，是受到每日动态压缩负荷。为了在这种环境中生存，常驻的软骨细胞样细胞必须能够控制它们的细胞体积，同时也控制它们的细胞外基质的合成代谢和分解代谢。最近的研究表明，在关节软骨的软骨细胞内表达一系列双向、跨膜的水和溶质转运蛋白，称为水通道蛋白（AQPs）。本研究的目的是利用免疫组织化学方法研究水通道蛋白1、2和3在人IVD中的表达。结果表明，NP和纤维内环细胞均表达AQP-1和-3，而AF外细胞缺乏AQP-1表达，AQP-3免疫阳性细胞数量极低。所有区域的细胞AQP-2均为阴性。因此，本研究证明了NP细胞和关节软骨细胞在表型上的相似性，这可能是由于组织渗透压和机械生物学的相似性。AQPs从NP到AF外层表达的减少可能表明，凝胶状NP和纤维状AF之间的机制生物学、渗透压和水合作用差异导致了细胞表型的变化。
The nucleus pulposus (NP) of the human intervertebral disc (IVD) is a hyperosmotic tissue that is subjected to daily dynamic compressive loads. In order to survive within this environment the resident chondrocyte-like cells must be able to control their cell volume, whilst also controlling the anabolism and catabolism of their extra-cellular matrix. Recent studies have demonstrated expression of a range of bi-directional, transmembrane water and solute transporters, named aquaporins (AQPs), within chondrocytes of articular cartilage. The aim of this study was to use immunohistochemsitry to investigate the expression of aquaporins 1, 2 and 3 within the human IVD. Results demonstrated expression of both AQP-1 and -3 by cells within the NP and inner annulus fibrosus (AF), while outer AF cells lacked expression of AQP-1 and showed very low numbers of AQP-3 immunopositive cells. Cells from all regions were negative for AQP-2. Therefore this study demonstrates similarities in the phenotype of NP cells and articular chondrocytes, which may be due to similarities in tissue osmolarity and mechanobiology. The decrease in expression of AQPs from the NP to the outer AF may signify changes in cellular phenotype in response to differences in mechanbiology, osmolarity and hydration between the gelatinous NP and the fibrous AF.