Redesigning Photosynthetic Membranes: Development of Bio-Inspired Photonic Nanomaterials
Redesigning Photosynthetic Membranes: Development of Bio-Inspired Photonic Nanomaterials
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重新设计光合膜：仿生光子纳米材料的开发
DOI：
10.1016/j.bpj.2015.11.159
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发表时间：
2016
影响因子：
3.4
通讯作者：
Adams P
中科院分区：
文献类型：
--
作者：
Adams P
生物细胞膜依靠层次组织来引发功能反应。在天然光合膜中，光收集（LH）膜蛋白复合物作为一个协调发色团的框架，吸收太阳能并将其引导到下游的生物能量过程。建立了支持脂质双分子层（slb）作为简单的模型膜，膜蛋白可以掺入其中。作为脂类的替代品，某些双嵌段共聚物可以从膜模拟系统中获得潜在的优势（增强的鲁棒性、功能性和响应性）。在这里，我们介绍了两种不同的生物启发LH系统的研究。首先，描述了一个模块化的人工LH系统，其中两亲性双嵌段聚合物聚（环氧乙烷）-嵌段聚（丁二烯）作为BODIPY能量供体和细菌氯能量受体发色团的非共价排列基质。聚合物/发色团复合材料在水溶液中形成纳米级胶束，在固体基底上形成无缺陷的单层和双层薄膜。稳态和时间分辨荧光光谱显示了供体-受体福斯特共振能量转移，并通过理论计算对系统进行了建模。负载型聚合物双分子层的能量传递效率高达90%。其次，我们介绍了正在进行的重新设计蛋白质/脂质LH系统的研究。纯化的植物蛋白和脂质被用作构建块，形成具有明确成分和3-D组织的新型重构蛋白/ slb，使用表面图案和光刻技术的组合。原子力、荧光显微镜和光谱学显示蛋白质排列和光收集功能是可以控制的。这些新的蛋白质/发色团和聚合物/发色团生物启发系统可以作为研究膜自组装和组织的平台，并可能导致基于芯片的纳米器件的应用。
Biological cell membranes rely upon hierarchical organization to elicit functional responses. In natural photosynthetic membranes, light harvesting (LH) membrane protein complexes act as a framework for coordination of chromophores which absorb solar energy and channel it to downstream bioenergetic processes. Supported lipid bilayers (SLBs) are established as simple model membranes, into which membrane proteins can be incorporated. As an alternative to lipids, certain diblock copolymers can from membrane-mimetic systems with potential advantages (increased robustness, functionality, responsivity). Here, we present research into two different bio-inspired LH systems.Firstly, a modular, artificial LH system is described, where amphiphilic diblock polymers, poly (ethylene oxide)-block-poly (butadiene), act a matrix for noncovalent arrangement of BODIPY energy donor and bacteriochlorin energy acceptor chromophores. The polymer/chromophore composites form nanoscale micelles in aqueous solution and defect-free monolayer and bilayer films on solid substrates. Donor-acceptor Forster resonance energy transfer is shown by steady state and time-resolved fluorescence spectroscopy and the system is modelled by theoretical calculations. Supported polymer bilayers demonstrated energy transfer efficiency up to 90%. Secondly, we present ongoing research into the redesign of protein/lipid LH systems. Purified plant proteins and lipids are used as building blocks to form novel reconstructed protein/SLBs with defined compositions and 3-D organization using a combination of surface patterning and photolithography techniques. Atomic force and fluorescence microscopy and spectroscopy show protein arrangement and light harvesting functionality can be controlled. These new protein/chromophore and polymer/chromophore bio-inspired systems could act as a platforms to investigate membrane self-assembly and organization and could lead to applications in chip-based nanodevices.