Localization of CD26/DPPIV in nucleus and its nuclear translocation enhanced by anti-CD26 monoclonal antibody with anti-tumor effect.
Localization of CD26/DPPIV in nucleus and its nuclear translocation enhanced by anti-CD26 monoclonal antibody with anti-tumor effect.
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DOI：
10.1186/1475-2867-9-17
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发表时间：
2009-06-26
影响因子：
5.8
通讯作者：
Yamada T
中科院分区：
文献类型：
--
作者：
Yamada K;Hayashi M;Du W;Ohnuma K;Sakamoto M;Morimoto C;Yamada T
CD 26是一种II型细胞表面糖蛋白，称为二肽基肽酶（DPP）IV。先前的研究已经揭示了CD 26在T细胞白血病/淋巴瘤和恶性间皮瘤中的表达，以及抗CD 26单克隆抗体（mAb）在体外和体内对CD 26+癌细胞生长的抑制作用。CD 26在肿瘤发展中的功能尚不清楚，CD 26 mAb诱导其抗肿瘤作用的机制仍不清楚。通过生化和免疫电镜分析显示，CD 26定位于T细胞白血病/淋巴瘤细胞和间皮瘤细胞的细胞核中。DPPIV酶活性在T细胞白血病/淋巴瘤细胞的核部分中被揭示。这些核内CD 26的表达通过用CD 26 mAb 1F 7处理而增强，对CD 26 + T细胞白血病/淋巴瘤细胞具有抗肿瘤作用。与此相反，CD 26 mAb，5 F8，没有抗肿瘤作用，不增加细胞核中的CD 26表达。生物素标记的细胞表面CD 26不断地易位到细胞核中，并且这种易位在1F 7处理下增强，但在5 F8处理下不增强。这些结果表明，细胞核内的CD 26从质膜移动，可能在人类癌细胞的生长中发挥一定的作用。
CD26 is a type II, cell surface glycoprotein known as dipeptidyl peptidase (DPP) IV. Previous studies have revealed CD26 expression in T cell leukemia/lymphoma and malignant mesothelioma, and an inhibitory effect of anti-CD26 monoclonal antibody (mAb) against the growth of CD26+ cancer cells in vitro and in vivo. The function of CD26 in tumor development is unknown and the machinery with which the CD26 mAb induces its anti-tumor effect remains uncharacterized. The localization of CD26 in the nucleus of T cell leukemia/lymphoma cells and mesothelioma cells was shown by biochemical and immuno-electron microscopic analysis. The DPPIV enzyme activity was revealed in the nuclear fraction of T cell leukemia/lymphoma cells. These expressions of intra-nuclear CD26 were augmented by treatment with the CD26 mAb, 1F7, with anti-tumor effect against the CD26+ T cell leukemia/lymphoma cells. In contrast, the CD26 mAb, 5F8, without anti-tumor effect, did not augment CD26 expressions in the nucleus. Biotin-labeled, cell surface CD26 translocated into the nucleus constantly, and this translocation was enhanced with 1F7 treatment but not with 5F8. These results indicate that the intra-nuclear CD26 which moves from plasma membrane may play certain roles in cell growth of human cancer cells.
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影响因子：
21.3
作者：
Lin, SY;Makino, K;Hung, MC
通讯作者：
Hung, MC
影响因子：
11.5
作者：
Inamoto, Teruo;Yamochi, Tadanori;Morimoto, Chikao
通讯作者：
Morimoto, Chikao
影响因子：
11.2
作者：
Wesley, UV;McGroarty, A;Homoyouni, A
通讯作者：
Homoyouni, A
影响因子：
6.4
作者：
Ohnuma, K;Ishii, T;Morimoto, C
通讯作者：
Morimoto, C
影响因子：
5.3
作者：
Giri, DK;Ali-Seyed, M;Hung, MC
通讯作者：
Hung, MC