DEVELOPMENT OF SEPTAL REGION IN RAT .1. NEUROGENESIS EXAMINED WITH THYMIDINE-H-3 AUTORADIOGRAPHY
DEVELOPMENT OF SEPTAL REGION IN RAT .1. NEUROGENESIS EXAMINED WITH THYMIDINE-H-3 AUTORADIOGRAPHY
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DOI：
10.1002/cne.901830108
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发表时间：
1979-01-01
影响因子：
2.5
通讯作者：
BAYER, SA
中科院分区：
文献类型：
--
作者：
BAYER, SA
用 3 H-胸苷放射自显影检查大鼠间隔区域的神经发生。产前组中的大鼠是怀孕雌性的后代，在重叠系列中连续几天注射 2 次 3H-胸苷：胚胎日 (E) 13 + E14、E14 + E15、...E21 + E22。出生后组中的大鼠以不重叠的系列进行注射：出生当天和出生后第1天、P2+P3、P3+P4。 60日龄时，在中线核组（斜带核、内侧和三角间隔核）、外侧间隔核和腹外侧核组（伏隔核、终纹床核和前连合）内的几个解剖水平测定标记细胞的百分比和每天形成期间添加的细胞比例。每个核组内的神经元形成显着不同的波，中线组的神经元在E13-E17之间形成，外侧间隔核在E15-E19之间形成，终纹床核和E14-E18之间的前连合以及E17-P2之间的伏隔核形成。所有细胞核和细胞核基团都显示出形成的特征梯度。中线核群和终纹床核（包括连合床核）的最早形成的神经元位于前连合交叉点附近。较年轻的神经元位于吻端和尾部，最年轻的神经元位于对角带核和纹状床核的最延端。在中线核组中的神经发生几乎完成后，外侧间隔核在其整个长度上沿着强的内侧梯度形成。在整个伏隔核的长度中，最古老的神经元位于腹侧，而逐渐年轻的细胞位于侧脑室下角下方的背侧。
Neurogenesis in the rat septal region was examined with 3H-thymidine autoradiography. The rats in the prenatal groups were the offspring of pregnant females given 2 injections of 3H-thymidine on consecutive days in an overlapping series: embryonic day (E) 13 + E14, E14 + E15,...E21 + E22. The rats in the postnatal groups were injected in a nonoverlapping series: the day of birth and postnatal day (P) 1, P2 + P3, P3 + P4. On 60 days of age, the percentage of labeled cells and the proportion of cells added during each day of formation were determined at several anatomical levels within the midline nuclear group (nucleus of the diagonal band, medial and triangular septal nuclei), the lateral septal nucleus and the ventrolateral nuclear group (nucleus accumbens, bed nuclei of the stria terminalis and the anterior commissure). The neurons within each nuclear group form in significantly different waves, those of the midline group forming between E13-E17, the lateral septal nucleus between E15-E19, the bed nuclei of the stria terminalis and anterior commissure between E14-E18 and the nucleus accumbens between E17-P2. All nuclei and nuclear groups show characteristic gradients of formation. Both the midline nuclear group and the bed nucleus of the stria terminalis (including the commissural bed nucleus) have their earliest forming neurons lying near the crossing of the anterior commissure. Younger neurons are located both rostrally and caudally with the youngest neurons lying in the most rostral extension of the diagonal band nucleus and the strial bed nucleus. The lateral septal nucleus forms along a strong mediolateral gradient throughout its length after neurogenesis is almost complete in the midline nuclear group. Throughout the length of the nucleus accumbens, the oldest neurons are located ventrally while progressively younger cells are found dorsally beneath the inferior horn of the lateral ventricle.