Bacteriological investigation on biopsy specimens from patients with sarcoidosis.
Bacteriological investigation on biopsy specimens from patients with sarcoidosis.
复制标题
结节病患者活检标本的细菌学调查。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
H. Okano
中科院分区：
文献类型：
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作者：
Homma Jy;C. Abe;H. Chosa;K. Ueda;J. Saegusa;M. Nakayama;Hiomi Homma;M. Washizaki;H. Okano
将在无菌条件下取出的活检标本（主要是淋巴结）乳化，并将匀浆接种到各种培养基中，然后进行有氧或厌氧培养。只有痤疮丙酸杆菌的分离率较高，细菌的最高浓度为6.5 × 10（5）/g组织：40个淋巴结中的28个和31个在固体和液体培养基上分别为阳性。痤疮丙酸杆菌也从对照非结节病淋巴结中分离出来：14个中的4个和8个分别在固体和液体培养基上呈阳性。细菌的最高浓度为1 × 10（4）。来自痤疮丙酸杆菌的细菌组分用于被动血凝试验，以估计抗细菌的抗体，也用于患者的皮肤试验。这些结果显示结节病患者和对照组患者之间仅有轻微差异。未分离出其他细菌或真菌。
Biopsy specimens, mostly lymph nodes, removed under aseptic conditions, were emulsified and the homogenate was inoculated in various media, which were incubated aerobically or anaerobically. Only Propionibacterium acnes was isolated in high incidence and the highest concentration of bacteria was 6.5 X 10(5) per g tissue: 28 and 31 out of 40 lymph nodes were positive on solid and on liquid media, respectively. P.acnes was also isolated from control non-sarcoidosis lymph nodes: 4 and 8 out of 14 were positive on solid and on liquid media, respectively. The highest concentration of bacteria was 1 X 10(4). A bacterial component derived from P. acnes was used for the passive hemagglutination test to estimate antibodies against the bacterium and also for skin test on patients. These results showed only slight differences between patients with sarcoidosis and control patients. No other bacteria or fungi were isolated.