Direct reprogramming of terminally differentiated B cells into erythroid lineage
Direct reprogramming of terminally differentiated B cells into erythroid lineage
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DOI：
10.1016/j.febslet.2012.08.019
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发表时间：
2012-10
期刊：
影响因子：
3.5
通讯作者：
K. Sadahira;Y. Fukuchi;Hiroyoshi Kunimono;M. Sakurai;Y. Ikeda;S. Okamoto;H. Nakajima
中科院分区：
文献类型：
--
作者：
K. Sadahira;Y. Fukuchi;Hiroyoshi Kunimono;M. Sakurai;Y. Ikeda;S. Okamoto;H. Nakajima
造血祖细胞已被证明可以保持可塑性，并通过适当的刺激转换谱系。然而，成熟的血细胞几乎没有表现出这种分化可塑性。在本文中，我们试图通过表达各种造血转录因子来将成熟的B细胞重新编程为红系。在众多因子中，GATA-1、SC1和CCAAT/增强子结合蛋白(C/EBP)α被证明是一组最小的因子，它们能有效地将终末分化的成熟B细胞重新编程为红系，克隆形成试验和红系特异性基因的表达证明了这一点。这项研究为从外周B细胞生成自体红细胞开辟了一条途径。
Hematopoietic progenitors have been shown to retain plasticity and switch lineages by appropriate stimuli. However, mature blood cells hardly showed such differentiation plasticity. In this paper, we tried to reprogram mature B cells into erythroid lineage by expressing various hematopoietic transcription factors. Among various factors, GATA-1, SCL together with CCAAT/enhancer binding protein (C/EBP) α turned out to be a minimal set of factors that efficiently reprogrammed terminally differentiated mature B cells into erythroid lineage, as evidenced by colony forming assays and erythroid-specific gene expressions. This study sets an avenue to generate autologous erythrocytes from peripheral B cells.