CULTURED RAT HEPATIC SINUSOIDAL ENDOTHELIAL-CELLS EXPRESS INTERCELLULAR-ADHESION MOLECULE-1 (ICAM-1) BY TUMOR-NECROSIS-FACTOR-ALPHA OR INTERLEUKIN-1-ALPHA STIMULATION
CULTURED RAT HEPATIC SINUSOIDAL ENDOTHELIAL-CELLS EXPRESS INTERCELLULAR-ADHESION MOLECULE-1 (ICAM-1) BY TUMOR-NECROSIS-FACTOR-ALPHA OR INTERLEUKIN-1-ALPHA STIMULATION
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DOI：
10.1016/s0168-8278(05)80142-4
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发表时间：
1994-06-01
影响因子：
25.7
通讯作者：
TANIKAWA, K
中科院分区：
文献类型：
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作者：
OHIRA, H;UENO, T;TANIKAWA, K
本研究调查了肿瘤坏死因子-α 或白细胞介素-1 α 刺激期间培养的大鼠肝窦内皮细胞上细胞间粘附分子-1（一种白细胞粘附分子）的表达。使用免疫电镜和针对细胞间粘附分子-1的免疫金技术，显示与未刺激的细胞相比，用肿瘤坏死因子-α（100 U/ml）或白细胞介素-1α：（10 U/ml）处理8小时的鼻窦上皮细胞表面金颗粒显着增加。此外，利用细胞荧光计对窦内皮细胞的细胞间粘附分子1进行半定量分析。即使没有刺激，细胞间粘附分子-1的表达也很弱。然而，肿瘤坏死因子-a或白细胞介素-1a处理后8小时，细胞上细胞间粘附分子-1的表达量呈剂量依赖性增加。动力学分析显示，这些细胞因子处理后的窦内皮细胞上细胞间粘附分子-1的表达从刺激开始到24 h逐渐增加。这些发现表明，肝窦内皮细胞可能通过表达白细胞粘附分子（例如细胞间粘附分子-1）介导白细胞与肝窦内皮细胞之间的直接相互作用。 (C) 肝病学杂志。
This study investigated the expression of intercellular adhesion molecule-1, a leukocyte adhesion molecule, on cultured rat hepatic sinusoidal endothelial cells during stimulation with tumor necrosis factor-alpha or interleukin-1 alpha. Using immunoelectron microscopy and the immunogold technique against intercellular adhesion molecule-1, gold particles were shown to increase significantly on the surface of sinusoidal epithelial cells treated with tumor necrosis factor-alpha (100 U/ml) or interleukin-1 alpha:(10 U/ml) for 8 h compared with unstimulated cells. In addition, semi-quantitative analysis of intercellular adhesion molecule-1 on the sinusoidal endothelial cells was performed by cytofluorometer. Even without stimulation, intercellular adhesion molecule-1 was weakly expressed. However, 8 h after tumor necrosis factor-a or interleukin-la treatment, the expression of intercellular adhesion molecule-1 on cells was increased in a dose-dependent manner. Kinetic analysis showed that the expression of intercellular adhesion molecule-1 on sinusoidal endothelial cells treated with these cytokines increased gradually from the beginning of stimulation to 24 h. These findings suggest that hepatic sinusoidal endothelial cells may mediate the direct interaction between leukocytes and sinusoidal endothelial cells by expressing leukocyte adhesion molecules such as intercellular adhesion molecule-1. (C) Journal of Hepatology.