Development of a reproducible method of analysis of iron, zinc and phosphorus in vegetables digests by SEC-ICP-MS
Development of a reproducible method of analysis of iron, zinc and phosphorus in vegetables digests by SEC-ICP-MS
复制标题
DOI：
10.1016/j.foodchem.2019.125652
复制
发表时间：
2020-03-05
期刊：
影响因子：
8.8
通讯作者：
Shewry, Peter R.
中科院分区：
文献类型：
--
作者：
Dell'Aquila, Caterina;Neal, Andrew L.;Shewry, Peter R.
蔬菜含有铁、锌和磷，与植酸盐形成复合物，限制了它们在素食中的可用性，这意味着非血红素缺铁性贫血和缺锌免疫功能障碍是一种风险。虽然这些元素已经分析了以前在生物液体和谷物使用LC-ICP-MS，有没有方法适合于分析铁，锌和磷同时在蔬菜中，因为它们的复杂的矩阵。在这项研究中，我们分析了铁，锌和磷的卷心菜，花椰菜，辣椒，菠菜，羽衣甘蓝和火箭后，模拟胃肠道消化使用新优化的SEC-ICP-MS方法。硝酸铵作为移动的相和合适的冲洗方案，允许良好的再现性和设备的维护。该方法具有良好的重现性，可以很容易地适应其他蔬菜，根据需要。
Vegetables contain iron, zinc and phosphorus as complexes with phytates limiting their availability from a vegetarian diet, meaning non-haem iron deficiency anaemia and zinc deficiency immune malfunction are a risk. Although these elements have been analysed previously in biological fluids and cereal using LC-ICP-MS, there is no method suitable for analysing iron, zinc and phosphorus simultaneously in vegetables because of their complex matrix. In this study, we analysed iron, zinc and phosphorus in cabbage, broccoli, pepper, spinach, kale and rocket after a simulated gastrointestinal digestion using a newly optimised SEC-ICP-MS method. Ammonium nitrate, as the mobile phase, and a suitable rinsing regime, allowed good reproducibility and maintenance of the equipment. The method showed good reproducibility and can be easily adapted to other vegetables, as required.