Identification of polymorphic DNA markers in cultivated peanut (Arachis hypogaea L.)
Identification of polymorphic DNA markers in cultivated peanut (Arachis hypogaea L.)
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DOI：
10.1023/a:1002949813052
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发表时间：
1997-01-01
期刊：
影响因子：
1.9
通讯作者：
Prakash, CS
中科院分区：
文献类型：
--
作者：
He, GH;Prakash, CS
本研究首次报道了栽培花生（ArachishypogaeaL.）DNA多态性的检测。测试了 DNA 扩增指纹识别 (DAF) 和扩增片段长度多态性 (AFLP) 方法检测花生遗传变异的潜力。 AFLP 方法更有效，因为 43% 的引物组合检测到多态性 DNA 标记，而 DAF 方法只有 3% 检测到多态性 DNA 标记。然而，使用两种方法中选择的引物鉴定的多态性带的数量是相当的。在DAF研究中，当筛选了559个不同类型的引物时，17个（主要是10聚体类型）检测到多态性，每个引物平均产生3.7个多态性条带，总共63个多态性标记。在AFLP研究中，当筛选了与限制性内切酶Eco RI和Mse I对应的64个引物组合（三个选择性核苷酸）时，有28个检测到多态性。平均而言，从这 28 个引物对获得的条带中有 6.7% 是多态性的，总共有 111 个 AFLP 标记。我们的结果表明，AFLP 和 DAF 方法均可用于生成花生 DNA 标记，因此在标记辅助遗传改良和这种重要经济作物的种质评估中具有潜力。
The detection of DNA polymorphism in cultivated peanut (Arachis hypogaea L.) is reported here for the first time. The DNA amplification fingerprinting (DAF) and amplified fragment length polymorphism (AFLP) approaches were tested for their potential to detect genetic variation in peanut. The AFLP approach was more efficient as 43% of the primer combinations detected polymorphic DNA markers in contrast to 3% with the DAF approach. However, the number of polymorphic bands identified using primers selected in both approaches was comparable. In the DAF study, when 559 primers of varying types were screened, 17 (mostly 10-mer types) detected polymorphism producing an average of 3.7 polymorphic bands per primer with a total of 63 polymorphic markers. In the AFLP study, when 64 primer combinations (three selective nucleotides) corresponding to restriction enzymes Eco RI and Mse I were screened, 28 detected polymorphism. On an average, 6.7% of bands obtained from these 28 primer pairs were polymorphic resulting in a total of 111 AFLP markers. Our results demonstrate that both AFLP and DAF approaches can be employed to generate DNA markers in peanut and thus have potential in the marker-assisted genetic improvement and germplasm evaluation of this economically important crop.