Differences in Mast Cell Infiltration, E-cadherin, and Zonula Occludens-1 Expression Between Patients With Overactive Bladder and Interstitial Cystitis/Bladder Pain Syndrome
Differences in Mast Cell Infiltration, E-cadherin, and Zonula Occludens-1 Expression Between Patients With Overactive Bladder and Interstitial Cystitis/Bladder Pain Syndrome
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DOI：
10.1016/j.urology.2012.01.047
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发表时间：
2012-07-01
期刊：
影响因子：
2.1
通讯作者：
Kuo, Hann-Chorng
中科院分区：
文献类型：
--
作者：
Liu, Hsin-Tzu;Shie, Jia-Heng;Kuo, Hann-Chorng
目的探讨膀胱过度活动综合征（OAB）与间质性膀胱炎/膀胱痛综合征（IC/BPS）患者肥大细胞浸润及尿路上皮屏障功能相关蛋白分布的差异。方法对27例OAB患者、18例IC/BPS患者和19例对照组进行膀胱壁活检。免疫荧光染色定量评价膀胱壁连接蛋白E-cadherin、紧密连接蛋白occluden （ZO-1）和活化肥大细胞的表达。结果对照组尿路上皮和上皮下肥大细胞数量较少（平均±标准误差1.77±0.47）。OAB （4.00 +/- 0.55, P = 0.002）和IC/BPS （4.64 +/- 0.72, P = 0.000）组肥大细胞浸润显著增加。与OAB （13.46 +/- 1.32, P = 0.004）和对照组（14.55 +/- 2.08,P = 0.004）相比，IC/PBS中ZO-1的表达量（7.45 +/- 0.99）显著降低。与对照组（96.30 +/- 9.15,P = .001）相比，IC/BPS膀胱标本中E-cadherin的表达也显著降低（59.05 +/- 9.48）。OAB组与对照组膀胱E-cadherin、ZO-1水平无显著差异（P = 0.170、P = 0.763）。结论OAB和IC/BPS膀胱壁均可见肥大细胞浸润，但E-cadherin和ZO-1表达仅在IC/BPS膀胱中降低，提示OAB膀胱中尿路上皮屏障功能未受影响。泌尿外科80:22 - 25。e18, 2012年。(C) 2012爱思唯尔公司
OBJECTIVE To investigate the difference of infiltration of mast cells and the distribution of protein involved in the urothelial barrier function between patients with overactive bladder syndrome (OAB) and interstitial cystitis/bladder pain syndrome (IC/BPS).METHODS Bladder wall biopsies were performed in 27 patients with OAB, 18 patients with IC/BPS, and 19 controls. The expression of junction protein E-cadherin, tight junction protein zonula occluden (ZO-1), and activated mast cells in the bladder wall were evaluated quantitatively using immunofluorescence staining.RESULTS The numbers of mast cells in the urothelium and suburothelium areas were low in the control group (mean +/- standard error 1.77 +/- 0.47). A highly significant increase in mast cell infiltration was observed in OAB (4.00 +/- 0.55, P = .002) and IC/BPS specimens (4.64 +/- 0.72, P = .000). ZO-1 expression was significantly decreased in IC/PBS (7.45 +/- 0.99) compared with OAB (13.46 +/- 1.32, P = .004) and control bladder samples (14.55 +/- 2.08, P = .004). The E-cadherin expression was also significantly decreased in IC/BPS bladder samples (59.05 +/- 9.48) compared with the controls (96.30 +/- 9.15, P = .001). No significant difference was found in E-cadherin or ZO-1 levels between the OAB and control bladders (P = .170 and P = .763, respectively).CONCLUSION Mast cell infiltration was found in both OAB and IC/BPS bladder wall, but E-cadherin and ZO-1 expression was only decreased in IC/BPS, suggesting the urothelial barrier function was not affected in the OAB bladder. UROLOGY 80: 225.e13-225.e18, 2012. (C) 2012 Elsevier Inc.