The use of selected ion flow tube mass spectrometry to detect and quantify polyamines in headspace gas and oral air
The use of selected ion flow tube mass spectrometry to detect and quantify polyamines in headspace gas and oral air
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DOI：
10.1002/rcm.4340
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发表时间：
2009-12-01
影响因子：
2
通讯作者：
Ladouceur, Chelsea
中科院分区：
文献类型：
--
作者：
Ross, Brian M.;Babay, Slim;Ladouceur, Chelsea
多胺是一类脂肪族化合物，包括腐胺、尸胺、精胺和亚精胺。它们参与多种细胞过程，并涉及许多不同的病理生理机制。多胺是一种挥发性化合物，具有独特的气味，通常被认为是令人不快的。然而，它们丰度的测量仅限于存在于水相中的化合物。使用选择性离子流管质谱（SIFT-MS），我们已经证明了多胺与离子h30 +， NO+和O-2（+）反应形成独特的产物离子，使其水平在气相中被量化。精胺的低挥发性不允许通过SIFT-MS对该化合物进行广泛的分析，而尸胺和腐胺的粘附特性需要使用PTFE传输线和耦合器。我们的数据表明，在口腔空气和腐烂牛肌肉的顶空中都存在尸胺和腐胺，而在人类精液的顶空中发现了腐胺和亚精胺的产物离子。因此，SIFT-MS似乎是一种测量气相多胺水平的实用方法，在生物学、医学、牙科和食品科学中都有应用。版权所有John Wiley & Sons， Ltd. 2009
Polyamines are a class of aliphatic compounds which include putrescine, cadaverine, spermine and spermidine. They are involved in a variety of cellular processes and have been implicated in a number of different pathophysiological mechanisms. Polyamines are volatile compounds having a distinctive odour normally perceived as being unpleasant. The measurement of their abundance has, however, been restricted to compounds present in the aqueous phase. Using selected ion flow tube mass spectrometry (SIFT-MS) we have shown that the polyamines react with the ions H3O+, NO+ and O-2(+) to form distinctive product ions allowing their levels to be quantified in the vapour phase. The low volatility of spermine did not allow extensive analysis of this compound by SIFT-MS while the adherent properties of cadaverine and putrescine required the use of PTFE transfer lines and couplers. Our data suggested the presence of cadaverine and putrescine in both oral air and the headspace of putrefying bovine muscle, while product ions corresponding to putrescine and spermidine were found in the headspace of human semen. SIFT-MS therefore appears to be a practical means of measuring vapour-phase polyamine levels, having applications in biology, medicine and dentistry, and food science. Copyright (C) 2009 John Wiley & Sons, Ltd.