A prospective randomised study of laser reshaping of cartilage in vivo
A prospective randomised study of laser reshaping of cartilage in vivo
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DOI：
10.1007/pl00011365
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发表时间：
2001-01-01
影响因子：
2.1
通讯作者：
Lowe, J
中科院分区：
文献类型：
--
作者：
Jones, N;Sviridov, A;Lowe, J
利用低激光能量水平对软骨进行激光整形，通常是猪耳。在激光整形后4个月对耳朵进行检查，并通过摄影和从耳朵的藻酸盐印模中获得的模型来评估整形的稳定性。在3周、8周和4个月时对3只动物的软骨进行组织学研究，采用血红素和伊红（H&E）、周期性酸-希夫（PAS）和阿利新蓝染色以及Mib-1抗体染色检测周期细胞。这些耳朵与其他对照或未辐照的耳朵进行比较。所有接受治疗的耳朵最初都表现出形状的可控改变。其中四个在20 Hz的脉冲能量为2 J/cm(2)，光斑直径为1.1 mm下暴露7 s，并保持其形状长达14天。然而，暴露在相同能量水平下9 s的3例出现组织坏死，而治疗时间少于4 s的3例在24小时后恢复到原来的形状。组织学检查显示，在辐射中心外有明显的偏心软骨膜增生，明显没有任何炎症。在所有激光照射区域，PAS染色消失，软骨基质内阿利新蓝染色显著增加。照射后的软骨中没有存活的软骨细胞。用检测周期细胞的mb -1抗体染色显示，在新软骨产生的增殖的软骨膜周围细胞的小纤维作用中呈阳性染色。
Laser reshaping using low laser energy levels was performed on the cartilage often porcine ears. The ears were examined up to 4 months after laser reshaping and the stability of the reshaping was assessed by photography and casts obtained from alginate impressions of the ears. The cartilage was also studied histologically in three animals at 3 weeks, 8 weeks, and 4 months using haematoxylin and eosin (H&E), periodic-acid-Schiff (PAS) and alcian blue stains as well as Mib-1 antibody stain to detect cells in cycle. These ears were compared with the other control or non-irradiated ear. All the treated ears initially demonstrated a controlled alteration in their shape. Four were exposed to a pulse energy of 2 J/cm(2) at 20 Hz with a spot diameter of 1.1 mm for 7 s and retained their shape for up to 14 days. However, the three exposed to the same levels of energy for 9 s showed tissue necrosis whereas the three which were treated for less than 4 s returned to their original shape after 24 h. Histological examination revealed that there was a dramatic eccentric proliferation of perichondrium away ift-om the centre of irradiation with a noticeable absence of any inflammation. In all laser-irradiated areas there was a loss of staining with PAS and a dramatic increase in staining with alcian blue within the cartilage matrix. Viable chondrocytes were absent from the irradiated cartilage. Staining with a Mib-1 antibody that detects cells in cycle showed positive staining in a small fi-action of proliferated perichondrial cells where new cartilage had been produced.