Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary
Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary
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DOI：
10.3791/57794
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发表时间：
2018-06-01
影响因子：
1.2
通讯作者：
Masubuchi, Satoru
中科院分区：
文献类型：
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作者：
Komatsu, Kouji;Iwase, Akira;Masubuchi, Satoru
哺乳动物雌性在每个发情周期中周期性地排出几乎固定数量的卵母细胞。为了维持这种规律性和周期性，调节发生在下丘脑-垂体-性腺轴水平和卵巢中发育中的卵泡上。尽管研究活跃，但卵泡发育机制尚不清楚，因为从休眠的原始卵泡激活到排卵涉及几个步骤，并且由于每个卵泡阶段的调节复杂性不同。为了探讨卵泡发育的机制，以及整个发情周期中卵泡的动态变化，我们建立了小鼠卵巢组织培养模型，该模型可以在显微镜下观察卵泡发育。在培养的卵巢模型中均能再现系统卵泡发育、周期排卵和卵泡闭锁，培养条件可通过实验调节。在这里，我们证明了这种方法在研究卵泡发育和其他卵巢现象的调节机制中的有用性。
Mammalian females periodically ovulate an almost constant number of oocytes during each estrus cycle. To sustain such regularity and periodicity, regulation occurs at the hypothalamic-pituitary-gonadal axis level and on developing follicles in the ovary. Despite active studies, follicle development mechanisms are not clear because of the several steps involved from the dormant primordial follicle activation to ovulation, and because of the regulation complexity that differs at each follicular stage. To investigate the mechanisms of follicle development, and the dynamics of follicles throughout the estrus cycle, we developed a mouse ovarian tissue culture model that can be used to observe follicle development using a microscope. Systematic follicle development, periodical ovulation, and follicle atresia can all be reproduced in the cultured ovary model, and the culture conditions can be experimentally modulated. Here, we demonstrate the usefulness of this method in the study of the regulatory mechanisms of follicle development and other ovarian phenomena.