Royal jelly affects collagen crosslinking in bone of ovariectomized rats
Royal jelly affects collagen crosslinking in bone of ovariectomized rats
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DOI：
10.1016/j.jff.2014.01.019
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发表时间：
2014-03-01
影响因子：
5.6
通讯作者：
Uoshima, Katsumi
中科院分区：
文献类型：
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作者：
Kaku, Masaru;Rocabado, Juan Marcelo Rosales;Uoshima, Katsumi
皇家浆（RJ）是蜂王的必需食物，据报道它具有雌激素样活性。本研究的目的是评估RJ摄入量对骨质量的影响，重点是I型胶原蛋白的翻译后修饰。将RJ喂饲去卵巢大鼠12周。RJ摄入量不影响OVX诱导的股骨骨骺骨体积减少;然而，代表骨质量的一个方面的胶原交联（吡啶啉和脱氧吡啶啉）的减少显著减轻。在培养的MC 3 T3-E1成骨细胞中，RJ处理不影响细胞增殖、细胞分化、基质形成或矿化。然而，RJ处理刺激plods的表达，其编码赖氨酰羟化酶亚型，控制胶原交联途径，它也影响胶原交联。这些结果表明，口服摄入RJ可以通过调节I型胶原蛋白的翻译后修饰来改善骨质量。(C)2014爱思唯尔有限公司版权所有。
Royal jelly (RJ) is an essential food for queen bees, and it reportedly has estrogen-like activity. The objective of this study was to evaluate the effect of RJ intake on bone quality with a focus on the posttranslational modifications of type I collagen. RJ was fed to ovariectomized (OVX) rats for 12 weeks. RJ intake did not affect OVX-induced reduction in bone volume at the femur epiphysis; however, the reduction of collagen crosslinks (pyridinoline and deoxypyridinoline), which represent an aspect of bone quality, were significantly mitigated. In cultured MC3T3-E1osteoblasts, RJ treatment did not affect cell proliferation, cell differentiation, matrix formation, or mineralization. However, RJ treatment did stimulate expression of plods, which encode lysyl hydroxylase isoforms that control the collagen crosslinking pathway, and it also affected collagen crosslinking. These results indicate that oral intake of RJ could improve bone quality by modulating the posttranslational modification of type I collagen. (C) 2014 Elsevier Ltd. All rights reserved.