Synthesis of type II collagen in vitro by embryonic chick neural retina tissue.
Synthesis of type II collagen in vitro by embryonic chick neural retina tissue.
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胚胎鸡神经视网膜组织体外合成 II 型胶原。
DOI：
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发表时间：
1976
影响因子：
11.1
通讯作者：
D. Newsome
中科院分区：
文献类型：
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作者：
G. N. Smith;T. Linsenmayer;D. Newsome
体外培养的鸡视网膜神经组织产生的胶原具有II型胶原的特性。分离的29-32期鸡胚胎视网膜神经组织将[14C]甘氨酸和[~3H]脯氨酸掺入由α1链和较高分子量组成的胶原蛋白中。溴化氰消化胶原蛋白所产生的多肽与真正的软骨II型胶原蛋白产生的多肽在电荷性质上是相同的，用羧甲基纤维素层析法测定，用十二烷基硫酸钠-丙烯酰胺凝胶电泳法测定，大小分布是相同的。
Collagen produced by chick neural retina tissue in vitro has the properties of type II collagen. Isolated neural retina tissue from stage 29-32 chick embryos incorporated [14C]glycine and [3H]proline into collagen consisting of alpha1 chains and a higher molecular weight species. The peptides produced by cyanogen bromide digestion of the collagen are identical to those from authentic type II collagen from cartilage in charge properties, as determined by carboxymethyl-cellulose chromatography, and size distribution, as determined by sodium dodecyl sulfate-acrylamide gel electrophoresis.