Antibacterial peptide PR-39 affects local nitric oxide and preserves tissue oxygenation in the liver during septic shock.
Antibacterial peptide PR-39 affects local nitric oxide and preserves tissue oxygenation in the liver during septic shock.
复制标题
抗菌肽 PR-39 会影响局部一氧化氮，并在感染性休克期间维持肝脏组织氧合。
DOI：
10.1016/s0925-4439(02)00170-9
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
James,PhilipE
中科院分区：
文献类型：
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作者：
Madhani,Melanie;Barchowsky,Aaron;Klei,Linda;Ross,ChrisR;Jackson,SimonK;Swartz,HaroldM;James,PhilipE
研究了抗菌肽PR-39对内毒素血症小鼠模型中一氧化氮（NO）和肝氧合（pO 2）的影响。在体内电子顺磁共振（EPR）光谱被用来直接测量肝脏NO和pO 2。在肝组织内的两个不同解剖位置进行pO 2测量，以评估对血液供应（因此氧供应）和小叶氧合的影响;选择性地从肝窦或肝小叶的平均pO 2。PR-39诱导肝脏NO水平升高6 h后，注射脂多糖（LPS）作为一个结果，增加iNOS在肝脏中的表达，但对eNOS或循环NO代谢产物没有影响。PR-39治疗保留了窦式氧合，并改善了整个肝组织床的pO 2。我们认为PR-39在脓毒症模型中对肝脏的有益作用是通过增加局部NO水平和维持肝窦的氧供应来介导的。
The effects of the antibacterial peptide PR-39 on nitric oxide (NO) and liver oxygenation (pO2) in a mouse model of endotoxaemia have been explored. In vivo electron paramagnetic resonance (EPR) spectroscopy was used to make direct measurements of liver NO and pO2. Measurements of pO2were made at two different anatomical locations within hepatic tissue to assess effects on blood supply (hence oxygen supply) and lobule oxygenation; selectively from the liver sinusoids or an average pO2across the liver lobule. PR-39 induced elevated levels of liver NO at 6 h following injection of lipopolysaccharide (LPS) as a result of increased iNOS expression in liver, but had no effect on eNOS or circulatory NO metabolites. Sinusoidal oxygenation was preserved, and pO2across the hepatic tissue bed improved with PR-39 treatment. We propose that the beneficial effects of PR-39 on liver in this septic model were mediated by increased levels of local NO and preservation of oxygen supply to the liver sinusoids.