Size-dependent properties of M-PEIs nanogels for gene delivery in cancer cells
Size-dependent properties of M-PEIs nanogels for gene delivery in cancer cells
复制标题
用于癌细胞基因传递的 M-PEIs 纳米凝胶的尺寸依赖性特性
DOI：
10.1016/j.ijpharm.2007.01.050
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发表时间：
2007-06-29
影响因子：
5.8
通讯作者：
Dong, Li
中科院分区：
文献类型：
--
作者：
Xu, Dong-Mei;Yao, Si-De;Dong, Li
在水溶液中通过光- fenton反应制备不同粒径的聚亚胺纳米凝胶（M-PEIs），选取38、75、87、121、132和167 nm的样品进行体外转染。在A549、Be17402、BGC-823和Hela细胞中加载相同数量的质粒DNA （pLEGFP-C1）时，由于M-PEIs纳米凝胶结构均匀，组分相同，因此可以研究M-PEIs纳米凝胶的大小对基因转染效率的影响。利用光相关光谱和原子力显微镜对M-PEIs及其DNA配合物进行了表征。用流式细胞术和荧光显微镜观察蛋白表达情况。所有DNA复合物均无明显的细胞毒性，且在最佳质量比下表面带正电荷。因此，当使用相同数量的DNA转染细胞时，表达的蛋白受到m - pei大小的影响。此外，75 nm和87 nm的转染效率最高，约为30%，也是细胞系无关的。(c) 2007 Elsevier B.V.版权所有
Polyethyleneimine nanogels (named as M-PEIs) with different sizes were prepared by photo-Fenton reaction in aqueous solution from which samples of 38, 75, 87, 121, 132 and 167 nm were selected for in vitro transfection. The homogeneous structure and the same component made it possible to study the size effect of M-PEIs nanogels on gene transfection efficiency when loading the same quantity of plasmid DNA (pLEGFP-C1) into A549, Be17402, BGC-823 and Hela cells. M-PEIs and its DNA complexes were characterized by photo correlation spectroscopy and atomic force microscopy. The protein expression was observed by flow cytometry and fluorescence microscopy. All of the DNA complexes had no obvious cytotoxicity and the surface charges were positive charged at the optimum weight ratio. Therefore, the expressed protein was affected by the size of M-PEIs when the same quantity of DNA was used to transfect cells. In addition, the samples of 75 and 87 nm yielded the highest transfection efficiency about 30% in all of the four cell lines which were also cell line independent. (c) 2007 Elsevier B.V. All rights reserved.