Daphnetin inhibits invasion and migration of LM8 murine osteosarcoma cells by decreasing RhoA and Cdc42 expression
Daphnetin inhibits invasion and migration of LM8 murine osteosarcoma cells by decreasing RhoA and Cdc42 expression
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DOI：
10.1016/j.bbrc.2016.01.179
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发表时间：
2016-02-26
影响因子：
3.1
通讯作者：
Ashihara, Eishi
中科院分区：
文献类型：
--
作者：
Fukuda, Hiroki;Nakamura, Seikou;Ashihara, Eishi
祖师麻甲素，即7,8 - 二羟基香豆素，存在于金边瑞香的主要成分中，具有多种药理活性，包括对癌细胞的抗增殖作用。在本研究中，我们利用Transwell系统发现祖师麻甲素以剂量依赖的方式抑制高转移性小鼠骨肉瘤LM8细胞的侵袭和迁移。经过祖师麻甲素处理后，穿透Transwell膜的细胞比未处理的细胞更圆。免疫荧光分析显示祖师麻甲素减少了细胞内应力纤维和丝状伪足的数量。此外，祖师麻甲素处理显著降低了RhoA和Cdc42的表达水平。总之，二羟基香豆素衍生物祖师麻甲素抑制LM8细胞的侵袭和迁移，因此是一种有前景的用于治疗转移性癌症的药物。(C) 2016爱思唯尔公司。保留所有权利。
Daphnetin, 7,8-dihydroxycoumarin, present in main constituents of Daphne odora var. marginatai, has multiple pharmacological activities including anti-proliferative effects in cancer cells. In this study, using a Transwell system, we showed that daphnetin inhibited invasion and migration of highly metastatic murine osteosarcoma LM8 cells in a dose-dependent manner. Following treatment by daphnetin, cells that penetrated the Transwell membrane were rounder than non-treated cells. Immunofluorescence analysis revealed that daphnetin decreased the numbers of intracellular stress fibers and filopodia. Moreover, daphnetin treatment dramatically decreased the expression levels of RhoA and Cdc42. In summary, the dihydroxycoumarin derivative daphnetin inhibits the invasion and migration of LM8 cells, and therefore represents a promising agent for use against metastatic cancer. (C) 2016 Elsevier Inc. All rights reserved.