Progress in pH-Sensitive sensors: essential tools for organelle pH detection, spotlighting mitochondrion and diverse applications.
Progress in pH-Sensitive sensors: essential tools for organelle pH detection, spotlighting mitochondrion and diverse applications.
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pH敏感传感器的进展：细胞器pH检测的基本工具，焦点线粒体和不同的应用。
DOI：
10.3389/fphar.2023.1339518
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发表时间：
2023
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
pH敏感荧光蛋白彻底改变了细胞成像和生理学领域，提供了对基本细胞过程基础的动态pH变化的深入了解。这一全面的审查探讨了不同的应用和最新进展，在使用pH敏感的荧光蛋白。这些卓越的工具使研究人员能够可视化和监测亚细胞区室（特别是线粒体）内的pH变化，从而揭示细胞器特异性pH调节。它们在可视化突触传递中的胞吐和胞吞事件、监测细胞死亡和凋亡以及理解药物作用和疾病进展方面发挥关键作用。最近的进展已经导致光稳定性、pH特异性和亚细胞靶向的改善，从而增强了它们的效用。多重成像，三维可视化和超分辨率显微镜技术正在扩大pH敏感蛋白应用的范围。未来有望将其整合到光遗传学和药物发现中。随着其不断发展的能力，pH敏感荧光蛋白仍然是解开细胞动力学和推动生物研究突破的不可或缺的工具。这篇综述为寻求利用pH敏感荧光蛋白潜力的研究人员提供了一个全面的资源。
pH-sensitive fluorescent proteins have revolutionized the field of cellular imaging and physiology, offering insight into the dynamic pH changes that underlie fundamental cellular processes. This comprehensive review explores the diverse applications and recent advances in the use of pH-sensitive fluorescent proteins. These remarkable tools enable researchers to visualize and monitor pH variations within subcellular compartments, especially mitochondria, shedding light on organelle-specific pH regulation. They play pivotal roles in visualizing exocytosis and endocytosis events in synaptic transmission, monitoring cell death and apoptosis, and understanding drug effects and disease progression. Recent advancements have led to improved photostability, pH specificity, and subcellular targeting, enhancing their utility. Techniques for multiplexed imaging, three-dimensional visualization, and super-resolution microscopy are expanding the horizon of pH-sensitive protein applications. The future holds promise for their integration into optogenetics and drug discovery. With their ever-evolving capabilities, pH-sensitive fluorescent proteins remain indispensable tools for unravelling cellular dynamics and driving breakthroughs in biological research. This review serves as a comprehensive resource for researchers seeking to harness the potential of pH-sensitive fluorescent proteins.
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DOI：
10.1083/jcb.145.1.83
发表时间：
1999-04-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Infante C;Ramos-Morales F;Fedriani C;Bornens M;Rios RM
通讯作者：
Rios RM
影响因子：
5.1
作者：
Schulte A;Lorenzen I;Böttcher M;Plieth C
通讯作者：
Plieth C
影响因子：
4
作者：
Camara AKS;Zhou Y;Wen PC;Tajkhorshid E;Kwok WM
通讯作者：
Kwok WM
影响因子：
2.5
作者：
Fishilevich, Elane;Fitzpatrick, James A. J.;Minden, Jonathan S.
通讯作者：
Minden, Jonathan S.
影响因子：
4.5
作者：
Bachert, Collin;Fimmel, Claus;Linstedt, Adam D.
通讯作者：
Linstedt, Adam D.