The Genomic Sequence of the Oral Pathobiont Strain NI1060 Reveals Unique Strategies for Bacterial Competition and Pathogenicity.
The Genomic Sequence of the Oral Pathobiont Strain NI1060 Reveals Unique Strategies for Bacterial Competition and Pathogenicity.
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DOI：
10.1371/journal.pone.0158866
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Raes J
中科院分区：
文献类型：
--
作者：
Darzi Y;Jiao Y;Hasegawa M;Moon H;Núñez G;Inohara N;Raes J
菌株NI 1060是在小鼠结扎诱导的疾病模型中引起牙周炎的口腔细菌。为了更好地了解其致病性，我们确定了其2，553，982 bp基因组的完整序列。尽管与嗜肺巴斯德氏菌（一种基于其16S rRNA的肺炎相关啮齿动物）密切相关，但NI 1060基因组内容表明它们是通过替代代谢途径在不同能源上繁荣的不同物种。基因组和系统发育分析表明，菌株NI 1060是不同的属目前描述的巴斯德菌科，并可能代表一个新的物种。此外，我们还发现了参与脂寡糖合成、粘附素和细菌毒性蛋白的推定毒力基因。这些基因对于宿主适应和通过细菌竞争和致病性诱导生态失调具有潜在的重要性。重要的是，菌株NI 1060强烈刺激Nod1，一种先天免疫受体，但由于移码突变，在两个肽聚糖回收基因中存在缺陷。因此，对其基因组的深入分析为NI1060作为传染病主要模型系统的发展提供了重要见解。
Strain NI1060 is an oral bacterium responsible for periodontitis in a murine ligature-induced disease model. To better understand its pathogenicity, we have determined the complete sequence of its 2,553,982 bp genome. Although closely related to Pasteurella pneumotropica, a pneumonia-associated rodent commensal based on its 16S rRNA, the NI1060 genomic content suggests that they are different species thriving on different energy sources via alternative metabolic pathways. Genomic and phylogenetic analyses showed that strain NI1060 is distinct from the genera currently described in the family Pasteurellaceae, and is likely to represent a novel species. In addition, we found putative virulence genes involved in lipooligosaccharide synthesis, adhesins and bacteriotoxic proteins. These genes are potentially important for host adaption and for the induction of dysbiosis through bacterial competition and pathogenicity. Importantly, strain NI1060 strongly stimulates Nod1, an innate immune receptor, but is defective in two peptidoglycan recycling genes due to a frameshift mutation. The in-depth analysis of its genome thus provides critical insights for the development of NI1060 as a prime model system for infectious disease.