Carbon nanotube amplification strategies for highly sensitive immunodetection of cancer biomarkers
Carbon nanotube amplification strategies for highly sensitive immunodetection of cancer biomarkers
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DOI：
10.1021/ja062117e
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发表时间：
2006-08-30
影响因子：
15
通讯作者：
Rusling, James F.
中科院分区：
文献类型：
--
作者：
Yu, Xin;Munge, Bernard;Rusling, James F.
我们在本文中描述了使用单壁碳纳米管（SWNT）森林平台的电化学免疫传感器与多标记二级抗体-纳米管生物缀合物的组合，用于血清和组织裂解物中癌症生物标志物的高灵敏度检测。通过使用具有辣根过氧化物酶（HRP）标记和以高HRP/Ab（2）比率连接到碳纳米管（CNT）的二次抗体（Ab（2））的生物缀合物获得了极大放大的灵敏度。对于10 μ L未稀释小牛血清中的前列腺特异性抗原（PSA），该方法的检测限为4 pg mL（-1）（100阿莫尔mL（-1）），质量检测限为40 fg。通过与标准ELISA测定法进行比较，证明了人血清样品中PSA的准确检测。PSA定量测量前列腺组织样本中的PSA不能区分的金标准免疫组化染色方法。这些易于制造的单壁碳纳米管免疫传感器显示出对癌症生物标志物的临床筛选和即时诊断的良好前景。
We describe herein the combination of electrochemical immunosensors using single-wall carbon nanotube (SWNT) forest platforms with multi-label secondary antibody-nanotube bioconjugates for highly sensitive detection of a cancer biomarker in serum and tissue lysates. Greatly amplified sensitivity was attained by using bioconjugates featuring horseradish peroxidase (HRP) labels and secondary antibodies (Ab(2)) linked to carbon nanotubes (CNT) at high HRP/Ab(2) ratio. This approach provided a detection limit of 4 pg mL(-1) (100 amol mL(-1)), for prostate specific antigen (PSA) in 10 mu L of undiluted calf serum, a mass detection limit of 40 fg. Accurate detection of PSA in human serum samples was demonstrated by comparison to standard ELISA assays. PSA was quantitatively measured in prostate tissue samples for which PSA could not be differentiated by the gold standard immunohistochemical staining method. These easily fabricated SWNT immunosensors show excellent promise for clinical screening of cancer biomarkers and point-of-care diagnostics.