ENHANCEMENT OF DIAPAUSING FLESH FLY PUPARIA WITH ADDITIONAL HYDROCARBONS AND EVIDENCE FOR ALKANE BIOSYNTHESIS BY A DECARBONYLATION MECHANISM
ENHANCEMENT OF DIAPAUSING FLESH FLY PUPARIA WITH ADDITIONAL HYDROCARBONS AND EVIDENCE FOR ALKANE BIOSYNTHESIS BY A DECARBONYLATION MECHANISM
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DOI：
10.1016/0965-1748(92)90060-r
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发表时间：
1992-04-01
影响因子：
3.8
通讯作者：
KOLATTUKUDY, PE
中科院分区：
文献类型：
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作者：
YODER, JA;DENLINGER, DL;KOLATTUKUDY, PE
滞育果蝇幼虫体内的碳氢化合物是非滞育果蝇幼虫的两倍(约15对7-mU-g)。在苍蝇的整个生命过程中，表皮层碳氢化合物的数量增加，但只有在蛹阶段才能区分滞育和非滞育的队列。虽然部分额外的碳氢化合物沉积在幼虫的外表面，但大部分额外的碳氢化合物是在幼虫的内表面。少数在短日照条件下饲养而未能滞育的苍蝇也会产生大量碳氢化合物增强的幼虫，因此暗示碳氢化合物产量的增加并不总是与滞育的表达有关。温度升高可以增加非滞育苍蝇蛹中碳氢化合物的产生量，但与短日照(滞育)程序的影响相比，这种影响是温和的。此外，我们还证明，果蝇利用一种机制将脂肪酸转化为烷烃，该机制首先涉及将脂肪酸还原为醛(需要ATP、CoA和NADH作为辅助因子)，然后从醛中去除羰基(不需要辅助因子)。因此，我们首次提供了昆虫体内碳氢化合物合成涉及脱羰基的证据。
Puparia of diapausing flesh flies, Sarcophaga crassipalpis, are lined with twice as much hydrocarbon as their nondiapausing counterparts (ca 15 vs 7-mu-g). The quantity of epicuticular hydrocarbon increases throughout the life of the fly, but it is only in the pupal stage that a distinction can be made between diapausing and nondiapausing cohorts. Though some of the additional hydrocarbon is deposited on the exterior surface of the puparium, the bulk of the additional hydrocarbon is on the interior surface of the puparium. The few flies that fail to diapause when reared under short day conditions also produce puparia enhanced with an abundance of hydrocarbon, thus implying that the increase in production of hydrocarbons is not invariably linked to the expression of diapause. Elevation of temperature can increase the quantity of hydrocarbon produced in puparia from nondiapausing flies, but this effect is modest in comparison to the effect of short-day (diapause) programming. In addition, we demonstrate that flesh flies convert fatty acid to alkane utilizing a mechanism that first involves reduction of fatty acid to aldehyde (with ATP, CoA and NADH as required cofactors), then carbonyl removal from aldehyde (no cofactors needed). Thus, we provide the first evidence that hydrocarbon synthesis in insects involves decarbonylation.