A DNA tweezer-actuated enzyme nanoreactor
A DNA tweezer-actuated enzyme nanoreactor
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DOI：
10.1038/ncomms3127
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发表时间：
2013-07-01
影响因子：
16.6
通讯作者：
Yan, Hao
中科院分区：
文献类型：
--
作者：
Liu, Minghui;Fu, Jinglin;Yan, Hao
调节酶的功能对于调节细胞通路是必不可少的。在这里，我们报告了一种镊子状的DNA纳米设备来激活酶/辅因子对的活性。脱氢酶和NAD(+)辅因子连接在DNA镊子结构的不同臂上，通过在打开和关闭状态之间切换来实现酶功能的激活。酶/辅因子对在开放状态下是空间分离的，酶功能受到抑制，而在关闭状态下，酶通过两个分子的紧密接近而被激活。DNA镊子的构象状态是由添加的特定寡核苷酸控制的，这些寡核苷酸充当触发变化的热力学驱动力(燃料)。使用这种方法，成功地演示了几个外部控制的酶抑制和激活周期。响应性酶纳米器件的这一原理可用于调节其他类型的酶并引入反馈或前馈控制环。
The functions of regulatory enzymes are essential to modulating cellular pathways. Here, we report a tweezer-like DNA nanodevice to actuate the activity of an enzyme/cofactor pair. A dehydrogenase and NAD(+) cofactor are attached to different arms of the DNA tweezer structure and actuation of enzymatic function is achieved by switching the tweezers between open and closed states. The enzyme/cofactor pair is spatially separated in the open state with inhibited enzyme function, whereas in the closed state, enzyme is activated by the close proximity of the two molecules. The conformational state of the DNA tweezer is controlled by the addition of specific oligonucleotides that serve as the thermodynamic driver (fuel) to trigger the change. Using this approach, several cycles of externally controlled enzyme inhibition and activation are successfully demonstrated. This principle of responsive enzyme nanodevices may be used to regulate other types of enzymes and to introduce feedback or feed-forward control loops.