Experimental Leishmania major infection suppresses HIV-1 DNA vaccine induced cellular immune response.
Experimental Leishmania major infection suppresses HIV-1 DNA vaccine induced cellular immune response.
复制标题
实验性利什曼原虫主要感染抑制 HIV-1 DNA 疫苗诱导的细胞免疫反应。
DOI：
10.1159/000079992
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Boyer,JeanD
中科院分区：
文献类型：
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作者：
Robinson,TaraM;Nelson,Robin;Artis,David;Scott,Phillip;Boyer,JeanD
发展中国家的艾滋病流行是一场重大的全球危机，有效的疫苗势在必行。然而，许多寄生虫在发展中国家很常见，可能会导致慢性免疫激活状态，这种状态会偏向 Th2 型，并可能损害对疫苗或其他感染挑战的反应。在这项研究中，我们证明 BALB/c 小鼠的实验性利什曼原虫重大感染会抑制对基于 DNA 的 HIV-1 gag 疫苗的反应。 BALB/c 中的 L. Major 感染导致极化的 Th2 免疫反应。在这项研究中，用 HIV-1 gag DNA 疫苗免疫的幼稚 BALB/c 小鼠对疫苗抗原 HIV-1 gag 产生了细胞免疫反应。 CD8+ T 淋巴细胞能够在体外对 HIV-1 gag 刺激做出反应并分泌干扰素 (IFN)-γ。然而，在用 gag 抗原进行体外刺激后，感染硕大利斯特菌的、接种疫苗的 BALB/c 小鼠产生 IFN-γ 的 CD8+ T 细胞数量显着减少。这些数据表明，导致 Th2 特征的寄生虫感染会降低旨在诱导抗病毒 CD8+ T 细胞反应的 DNA 疫苗的功效。
The AIDS epidemic in the developing world represents a major global crisis and an effective vaccine is imperative. However, many parasites are common in developing countries and can result in a state of chronic immune activation that is polarized towards a Th2 profile and which can potentially impair responses to vaccines or other infectious challenges. In this study we demonstrate that experimental Leishmania major infection of BALB/c mice inhibits responses to a DNA-based HIV-1 gag vaccine. L. major infection in BALB/c results in a polarized Th2 immune response. In this study naïve BALB/c mice immunized with the HIV-1 gag DNA vaccine mounted a cellular immune response against the vaccine antigen, HIV-1 gag. CD8+ T lymphocytes were able to respond in vitro to HIV-1 gag stimulation and secrete interferon (IFN)-γ. However, L. major-infected, vaccinated BALB/c mice had a significantly reduced number of IFN-γ-producing CD8+ T cells following in vitro stimulation with gag antigen. These data suggest that parasitic infection, which results in a Th2 profile, reduces the efficacy of DNA vaccines that are designed to induce antiviral CD8+ T cell responses.