Control of cell attachment through polyDNA hybridization
Control of cell attachment through polyDNA hybridization
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DOI：
10.1016/j.biomaterials.2009.11.098
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发表时间：
2010-03-01
期刊：
影响因子：
14
通讯作者：
Iwata, Hiroo
中科院分区：
文献类型：
--
作者：
Teramura, Yuji;Chen, Hao;Iwata, Hiroo
细胞间的相互作用在胚胎发育和体内平衡的维持中起着至关重要的作用。对于基于细胞的组织工程和再生医学疗法，必须严格控制这种相互作用，并且正在使用生物医学和工程方法开发用于研究和控制细胞-细胞相互作用的方法。在这项研究中，我们制备了两亲性PEG-脂质聚合物，连接到具有特定序列的polyDNA。用聚DNA-PEG-脂质缀合物孵育细胞将一些聚DNA转移到细胞表面。类似地，将使用具有互补序列的聚DNA的聚DNA-PEG-脂质缀合物引入到其它细胞的表面或基底表面。随后通过两个互补的polyDNA之间的杂交介导细胞-细胞或细胞-基质附着，并使用荧光显微镜进行监测。(C)2009爱思唯尔有限公司保留所有权利。
Cell-cell interactions play vital roles in embryo development and in homeostasis maintenance. Such interactions must be stringently controlled for cell-based tissue engineering and regenerative medicine therapies, and methods for studying and controlling cell-cell interactions are being developed using both biomedical and engineering approaches. In this study, we prepared amphiphilic PEG-lipid polymers that were attached to polyDNA with specific sequences. Incubation of cells with the polyDNA-PEG-lipid conjugate transferred some of the polyDNA to the cells' surfaces. Similarly, polyDNA-PEG-lipid conjugate using polyDNA with a complementary sequence was introduced to the surfaces of other cells or to a substrate surface. Cell-cell or cell-substrate attachments were subsequently mediated via hybridization between the two complementary polyDNAs and monitored using fluorescence microscopy. (C) 2009 Elsevier Ltd. All rights reserved.