Microarray analysis of Arabidopsis plants in response to allelochemical L-DOPA
Microarray analysis of Arabidopsis plants in response to allelochemical L-DOPA
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DOI：
10.1007/s00425-010-1294-7
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发表时间：
2011-02-01
期刊：
影响因子：
4.3
通讯作者：
Fujii, Yoshiharu
中科院分区：
文献类型：
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作者：
Golisz, Anna;Sugano, Mami;Fujii, Yoshiharu
绒毛豆（Mucuna pruriens）植物阻碍邻近植物的生长。一种化合物，3-（3 '，4'-二羟基苯基）-l-丙氨酸（l-DOPA），负责绒豆的化感能力。该化合物是一种活性化感物质，可降低几种植物的根生长。在哺乳动物中，l-多巴是用于帕金森病症状缓解的众所周知的治疗剂。然而，它在植物中的作用方式仍然没有很好的理解。为了解决这些问题，基因表达拟南芥植物，已暴露于左旋多巴，使用DNA微阵列进行了分析。结果表明，在L-DOPA作用6 h后，110个基因表达显著上调，69个基因表达显著下调。这些诱导基因可以分为不同的功能类别，主要是基于亚细胞定位，代谢和蛋白质的结合功能或辅因子的要求。基于这些结果，我们认为，左旋多巴的行为有两种机制：它影响氨基酸代谢和失调金属稳态，特别是铁，这是所有生物体的基本生物过程所需的。
Velvetbean (Mucuna pruriens) plants impede the growth of neighboring plants. One compound, 3-(3',4'-dihydroxyphenyl)-l-alanine (l-DOPA), is responsible for the allelopathic capacity of velvetbean. This compound is an active allelochemical that decreases root growth of several plant species. In mammals, l-DOPA is a well-known therapeutic agent for the symptomatic relief of Parkinson's disease. However, its mode of action in plants is still not well understood. To address such issues, gene expression in Arabidopsis thaliana plants, which had been exposed to l-DOPA, was analyzed using DNA microarrays. After 6 h of l-DOPA exposure, the expression of 110 genes was significantly upregulated, and the expression of 69 genes was significantly downregulated. These induced genes can be divided into different functional categories, mainly on the basis of subcellular localization, metabolism, and proteins with a binding function or cofactor requirement. Based on these results, we suggest that l-DOPA acts by two mechanisms: it influences amino acid metabolism and deregulates metal homeostasis, especially that of iron, which is required for the fundamental biological processes of all organisms.