Arabidopsis CAPRICE-LIKE MYB 3 (CPL3) controls endoreduplication and flowering development in addition to trichome and root hair formation
Arabidopsis CAPRICE-LIKE MYB 3 (CPL3) controls endoreduplication and flowering development in addition to trichome and root hair formation
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DOI：
10.1242/dev.017947
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发表时间：
2008-04-01
期刊：
影响因子：
4.6
通讯作者：
Wada, Takuji
中科院分区：
文献类型：
--
作者：
Tominaga, Rumi;Iwata, Mineko;Wada, Takuji
CAPRICE（CPC）编码一个具有R3 MYB基序的小分子蛋白质，并促进拟南芥根毛细胞分化。三个额外的CPC样MYB基因TRY（TRIPTYCHON）、ETC 1（TRY和CPC 1的增强子）和ETC 2（TRY和CPC 2的增强子）以冗余方式与毛状体和根毛形成中的CPC起作用。在这项研究中，我们确定了一个额外的同源物，CPC样MYB 3（CPL 3），它与CPC，TRY，ETC 1和ETC 2具有高度的序列相似性。CPL 3的过表达导致毛状体的抑制和根毛的过量产生，如已经观察到的CPC、TRY、ETC 1和ETC 2。形态学研究与双重，三重和四重同源突变体表明，CPL 3基因协同调节表皮细胞分化与其他CPC同系物。启动子-GUS分析表明，CPL 3特异性表达于叶表皮细胞，包括气孔保卫细胞。值得注意的是，CPL 3基因通过调节核内复制对开花发育、表皮细胞大小和毛状体分支具有多效性作用。
CAPRICE (CPC) encodes a small protein with an R3 MYB motif and promotes root hair cell differentiation in Arabidopsis thaliana. Three additional CPC-like MYB genes, TRY (TRIPTYCHON), ETC1 (ENHANCER OF TRY AND CPC 1) and ETC2 (ENHANCER OF TRY AND CPC 2) act in a redundant manner with CPC in trichome and root hair patterning. In this study, we identified an additional homolog, CPC-LIKE MYB 3 (CPL3), which has high sequence similarity to CPC, TRY, ETC1 and ETC2. Overexpression of CPL3 results in the suppression of trichomes and overproduction of root hairs, as has been observed for CPC, TRY, ETC1 and ETC2. Morphological studies with double, triple and quadruple homolog mutants indicate that the CPL3 gene cooperatively regulates epidermal cell differentiation with other CPC homologs. Promoter-GUS analyses indicate that CPL3 is specifically expressed in leaf epidermal cells, including stomate guard cells. Notably, the CPL3 gene has pleiotropic effects on flowering development, epidermal cell size and trichome branching through the regulation of endoreduplication.